非小细胞肺癌中survivin蛋白的表达及其与 p53表达的关系_医学论文.docVIP

非小细胞肺癌中survivin蛋白的表达及其与 p53表达的关系_医学论文 非小细胞肺癌中survivin蛋白的表达及其与 p53表达的关系_医学论文 作者：王静， 杨继要， 石敏， 吴逸明 ，吴拥军 【摘要】 目的 探讨非小细胞肺癌组织中survivin蛋白的表达与临床病理特征的关系及其与p53表达的相关性。方法 采用免疫组化的链霉菌抗生物素过氧化酶法（Streptavidin）检测80例NSCLC组织和20例正常肺组织中survivin、p53蛋白表达情况。结果 80例非小细胞肺癌组织中survivin蛋白阳性例数49例，表达率为61.3%，在肺良性病变组织中，未阳性表达，两组比较差异有显著性（0.05）；在III～IV期肺癌组织中survivin蛋白阳性表达率显著高于I～II期肺癌组织(0.05)，而与组织学分型、分化程度、 淋巴结转移与否无关(Pgt0.05)；survivin、突变型p53蛋白在肺癌组织中的表达呈正相关。结论 survivin蛋白表达与肺癌的TNM分期密切相关，有可能成为判断病情和评价预后的指标，survivin有望成为肺癌诊断的肿瘤标志物及基因治疗的新靶点；survivin、突变型p53蛋白在肺癌组织的协同高表达可能促使肺癌病情的恶性发展。 【关键词】 非小细胞肺癌；survivin；p53；表达 Corresponding Author: Wu Yi：Objective To study the expression of survivin and its relationship with the clinical pathological features of the NSCLC(nonthods The expression of survivin,p53 protein was detected in 80 cases of NSCLC tissues and 20 cases of normal lung tissue by immunohistochemical StreptavidinPeroxidase (SP) method.Results survivin was expressed in 49 of 80(61.3%)cases of lung carcinoma and in 0 of 20(0) cases of normal lung tissues with the expression of survivin in lung carcinoma significantly higher than that in normal lung tissues(0.05).The positive rate of survivin in stage Ⅲ～Ⅳwas significantly higher than that of survivin in stage Ⅰ～Ⅱ (0.05)；There was no relationship between survivin gene expression and histological subtypes,differentiation，TNM stages,lymph node metastasis (Pgt0.05),There is the positive correlation existing in the expression of Survivin,mutation.Conclusion survivin with only expression in the cancer tissues,not in benign lung tissues，may play an important role in lung carcinogenesis and be identified as a definite diagnostic marker and a potential therapeutic target in NSCLC.Cooperative high expression of survivin and mutationP53 in lung cancer tissues may make lung cancer develop quickly. Key words：NSCLCsurvivinp53Expression 本实验采用免疫组化方法，比较了正常肺组织和肺癌组织中survivin、p53表达的差异,探讨survivin与非小细胞肺癌的组织学分型、分化程度、淋巴结转移的关系及其与p53表达的相关性，为今后可能以survivin基因作为靶点对非小细胞肺癌对进行基因治疗和预后评价提供理论依