高同型半胱氨酸血症对球囊损伤大鼠颈动脉核转录因子NF_药学论文.docVIP

高同型半胱氨酸血症对球囊损伤大鼠颈动脉核转录因子NF_药学论文 高同型半胱氨酸血症对球囊损伤大鼠颈动脉核转录因子NF_药学论文 作者：徐会圃，刘长梅，冯义柏 【关键词】 ,甲硫氨酸 Effect of homocysteine on expressions of NFκB and protooncogene in ballooninjured rat carotid arteries 　　【Abstract】 AIM: To study the effect of homocysteine (Hcy) on the expressions of nuclear factorκB (NFκB) P65 protein and cfos, cjun mRNA in common carotid artery of rats after balloon injury and to explore the possible mechanism by which homocysteine exacerbates neointimal hyperplasia after angioplasty. METHODS: The expressions of NFκB P65 protein and cfos and cjun mRNA were detected by Western blot and semiquantitative RTPCR respectively in the common carotid artery. RESULTS: The expressions of NFκB P65 protein and cfos and cjun mRNA were significantly higher in low methionine (LM) group（1.12±0.13, 1.40±0.21, 1.43±0.25）（0.05） and high methionine (HM) group（1.50±0.37, 1.68±0.27, 1.71±0.30）（0.01）than those in control group（0.64±0.06, 1.10±0.15, 1.00±0.13）. The expressions of NFκB P65 protein and cfos, cjun mRNA were also significant higher in HM group than those in LM group (0.05). CONCLUSION: Homocysteine may upregulate the transcription of cfos and cjun mRNA in common carotid artery after balloon angioplasty by NFκB pathway, which may be responsible for neointimal hyperplasia induced by homocysteine after angioplasty. 　　【Keywords】 methionine; homocysteine; balloon injury; NFkappaB; protooncogenes 　　【摘要】 目的： 观察高蛋氨酸饮食诱导的高同型半胱氨酸血症对大鼠颈动脉球囊损伤后血管组织NFκBP65蛋白和原癌基因cfos及cjun mRNA表达的影响，探讨同型半胱氨酸在血管球囊损伤后新内膜过度增生中的可能机制. 方法： 采用Western blot及RTPCR方法分别检测血管组织NFκBP65蛋白和cfos及cjun mRNA表达，并做半定量分析. 结果： 低、高蛋氨酸组血管组织NFκBP65蛋白、cfos及cjun mRNA的表达均高于对照组，分别为1.12±0.13 vs 0.64±0.06 （0.05）, 1.50±0.37 vs 0.64±0.06 (0.01), 1.40±0.21 vs 1.10±0.15 （0.05）, 1.43±0.25 vs 1.10±0.15 （0.01）及1.68±0.27 vs 1.00±0.13 （0.05）, 1.71±0.30 vs 1.00±0.13 （0.01）,高蛋氨酸组也明显高于低蛋氨酸组(0.05). 结论： 同型半胱氨酸有可能通过NFκB通路上调血管内皮损伤后动脉组织原癌基因cfos及cjun mRNA的表达，这可能是其促进血管内皮球囊损伤后新内膜过度增生的机制之一. 　　【关键词】 甲硫氨酸；同型半胱氨酸；球囊损伤；原癌基因；NFκB　　0引言 　　实验研究和临床观察表明，血中同型半胱