MBR系统CANON工艺的快速启动及微生物种群特征.pdfVIP

中国环境科学 2014,34(11) ：2788~2795 China Environmental Science MBR 系统 CANON 工艺的快速启动及微生物种群特征 李 冬 1*,何永平 1,张肖静 2,梁瑜海 1,张玉龙 1,范 丹 1 (1.北京工业大学水质科学与水环境恢复工程北京市 重点实验室,北京 100124 ；2.哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江 哈尔滨 150090) 摘要：为了考察 CANON 工艺的快速启动策略及功能微生物的种群特征,在常温 MBR 反应器内接种普通活性污泥后间歇运行.启动策略为 以调控曝气时间和曝气量作为主要方法,首先在限氧条件下启动亚硝化,之后进一步降低 DO 启动 CANON 工艺.在 CANON 工艺启动成功 后,通过调整曝气时间和无机碳源浓度提高了总氮去除负荷,并采用PCR-DGGE 技术分析了稳定运行的 CANON 工艺内功能微生物的种群 特征 . 结果表明,CANON 工艺经 36d 成功启动,NH4+-N 去除率和总氮去除率最终稳定在 99% 和 84% 左右, 氮去除负荷达到 0.41kg/(m3·d).DGGE 测序结果表明,Nitrosomonas 和 Candidatus Kuenenia stuttgartiensis 是反应器内的优势菌种,两种微生物协同作用,共同 在 MBR 内完成了高效的自养脱氮. 关键词：MBR ；普通活性污泥；CANON ；启动；PCR-DGGE ；微生物 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2014)11-2788-08 1* The fast start-up of CANON process in MBR system and the characterization of microbes. LI Dong , HE 1 2 1 1 1 Yong-ping , ZHANG Xiao-jing , LIANG Yu-hai , ZHANG Yu-long , FAN Dan (1.Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China；2.State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China). China Environmental Science, 2014,34(11)：2788~2795 Abstract ：To investigate the fast start-up of CANON process and characterization of functional microbes, conventional activated sludge was inoculated to an MBR and the reactor was operated intermediately at ambient temperature. In the launch strategy, the regulation of aeration and aeration time was used as the main method. First, partial nitrification was applied under oxygen-limited condition. Secondly, DO had to be decreased fu