两步培养法提高栅藻的生物量及油脂含量.pdfVIP

2014年第39卷第5期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 中摇 国摇 油摇 脂 53 微生物油脂 两步培养法提高栅藻的生物量及油脂含量 1 1 1,2,3 1,2,3 李小妹 ,廖兴辉 ,王明兹 ,陈必链 (1. 福建师范大学生命科学学院,福州350108;2. 福建师范大学工业微生物教育部工程研究中心, 福州350108;3. 福建省现代发酵技术工程研究中心,福州350108) 摘要:采用两步法培养栅藻,即在低光强-含氮条件下,培养栅藻至稳定期积累生物量,然后将栅藻 转移至高光强-缺氮培养基中积累油脂。 结果表明:在低光强-含氮培养基BG11(+N)条件下, 适宜栅藻生长的低光强为2000~2400 lx,培养期为10 d;转高光强-缺氮培养基BG11(-N)条件 下培养,适宜栅藻积累油脂的高光强为6000~6 500 lx,培养期为6 d。 利用两步法培养栅藻,其生 物量可达(0.957 依0.126) g/ L,油脂含量可达(23.35 依0.2)%。 两步法培养栅藻的生物量和油脂 含量分别比常规单步法培养(10 d)的提高了35.74%和近1倍。 关键词:栅藻;两步法培养;油脂含量;生物量 中图分类号:TS222;Q936摇 摇 文献标志码:A 摇 摇 文章编号:1003-7969(2014)05-0053-04 Improvement of biomass and oil content of Scenedesmus sp. by two-step cultivation 1 1 1,2,3 1,2,3 LI Xiaomei ,LIAO Xinghui ,WANG Mingzi ,CHEN Bilian (1. College of Life Sciences,Fujian Normal University,Fuzhou350108,China; 2.Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University,Fuzhou350108,China; 3. Center of Fujian Modern Fermentation Technology and Engineering,Fuzhou350108,China) Abstract:Scenedesmus sp. was cultured to stationary phase under the conditions of low illumination in鄄 tensity withnitrogen,andthenculturedinmediumwithhighilluminationintensity andnitrogendeficiency to accumulate oil. The results showed that in nitrogenous BG11medium with low illumination intensity, the optimal illumination intensity for the growth of Scenedesmus sp. was2000-2400 lx,culturingfor 10 d;in nitrogendeficiency BG11mediumwithhighilluminationintensity,thesuitableilluminationintensi鄄 ty foroilaccumulationofScenedesmus sp. was600