化学萃取的异种神经修复神经缺损的可行性研究_临床医学论文.docVIP

化学萃取的异种神经修复神经缺损的可行性研究_临床医学论文 化学萃取的异种神经修复神经缺损的可行性研究_临床医学论文 作者：刘品端，王伟，智晓东，梅晰凡 【摘要】 　　目的 探讨联合应用十二烷基硫酸钠（Sodium dodecyl sulfate，SDS）和脱氧胆酸钠（Sodium deoxycholate）的化学萃取方法，对异种神经移植段进行脱细胞处理，构建异种神经去细胞基质膜管，应用于修复周围神经缺损的可行性。方法 取新鲜兔坐骨神经，切成长10mm的神经段，用十二烷基硫酸钠（Sodium dodecyl sulfate，SDS）和脱氧胆酸钠（Sodium deoxycholate）反复处理新鲜的兔神经段构建成异种神经去细胞基质膜管,修复大鼠坐骨神经缺损。结果 4 个月后异种神经基质膜管与大鼠坐骨神经缝合处愈合良好，未见有神经瘤及粘连，HE染色显示异种神经基质膜管组桥接处再生神经结构连续性良好,移植段可见神经纤维及新生的血管。S-100免疫组织化学染色可见阳性的雪旺氏细胞沿神经纤维分布成条带状。电镜超微结构观察可见再生的神经纤维较粗大，排列紧密，呈均匀一致圆型或椭圆型，髓鞘较厚，轴突结构完整。 结论 联合应用十二烷基硫酸钠（Sodium dodecyl sulfate，SDS）和脱氧胆酸钠（Sodium deoxycholate）的化学萃取方法构建的异种神经去细胞基质膜管可以修复大鼠坐骨神经缺损。 【关键词】 异种神经 去细胞基质膜管 化学萃取 坐骨神经 大鼠 　　 　　Abstract: Objective To explore the feasibility of using the xenogeneic acellular nerve scaffold dealt with through new chemical extractive method which used Sodium dodecyl sulfate (SDS) and Sodium deoxycholate to restore the peripheral nerve defect. MethodsFresh sciatic nerve of rabbit was treated with a solution of SDS and a solution of sodium deoxycholate，which was used to restore the peripheral nerve defect. Results Four months after operation, the anastomosis of the xenogeneic acellular nerve scaffold was smooth, and there were no neuroma, conglutination, inflammation and immune reaction. HE Staining showed that there were a lot of new born fibers and regenerated myelinated nerve fibers in the xenogeneic acellular nerve under light microscopy. Positive Schwann cells with S-100 immune staining showed a strip-shaped distribution along the regenerated nerve fibers. The structure of axon was completed, and the arrangement of regenerated nerve fibers with rotund or elliptoid shapes was closed. Conclusions The xenogeneic acellular nerve dealt with through new chemical extractive method which used Sodium dodecyl sulfate (SDS) and Sodium deoxycholate can be applied in the restoration of peripheral nerve defect. 　　Key words：xenogeneic nerve; acellular nerve scaffold; chemic extraction; sciatic nerve; rat 　　周围神经缺损是临床上常见的损伤性疾病，当神经纤维断裂长度