化疗药物对TRAIL在急性白血病原代细胞表达的影响_临床医学论文.docVIP

化疗药物对TRAIL在急性白血病原代细胞表达的影响_临床医学论文 化疗药物对TRAIL在急性白血病原代细胞表达的影响_临床医学论文 作者：刘延方，陈胜梅，孙慧，董见喜，张秋堂【摘要】 为了探讨TRAIL在急性白血病原代细胞表达及化疗药物对TRAIL在急性白血病细胞表达的影响，采集16例初诊的急性白血病患者化疗前、化疗1天后、3天后和5天后的外周血，分离单个核细胞并用流式细胞术检测TRAIL的表达；同时采集12例初诊的急性白血病患者化疗前骨髓，分离单个核细胞进行培养，分别加VP-16和/或干扰素，培养一定时间后检测TRAIL的表达水平。结果表明：与化疗前相比，初治急性白血病患者外周血单个核细胞TRAIL的表达水平在化疗1天后即明显提高（0.05）；在体外培养实验中，VP-16上调TRAIL在急性白血病骨髓单个核细胞的表达(0.05)，VP-16联合干扰素与单独应用VP-16相比，TRAIL的表达水平无差异（Pgt;0.05）。结论：化疗药物治疗白血病的机制之一可能是通过诱导TRAIL的表达而促进白血病细胞的凋亡。 【关键词】 急性白血病 　　Influence of Chemotherapy on Expression of TRAIL in Primary Acute Leukemic Cells 　　Abstract In order to explore the expression of TRAIL in primary acute leukemic cells and the effect of chemotherapyeutic drug on TRAIL expression in acute leukemic cells，the expression of TRAIL was assessed by flow cytometry on day 0，day 1，day 3 and day 5 in 16 patients with acute leukemia received chemotherapy. Meanwhile，the bone marrow mononuclear cells of acute leukemia patients were cultured in vitro with VP-16 and INFα-2a. Expression of TRAIL was analyszed by flow cytometry at 24，48 and 72 hours after treatment. The results showed that the expression of TRAIL in the peripheral blood mononuclear cells was upregulated significantly from day 1 after chemotherapy(0.05). In in vitro culture test，VP-16 upregulated the expression of TRAIL on acute leukemia bone marrow mononuclear cells(0.05). Compared with VP-16 alone，the combination of VP-16 with IFNα-2a showed no synergic effects on the expression of TRAIL. It is concluded that the expression of TRAIL increases after chemotherapy in vivo and after treatment with VP-16 and IFN in vitro，which suggests that the apoptosis induced by TRAIL may play an important role in chemotherapy of leukemia. 　　Key words TRAIL; acute leukemia; chemotherapy drug 细胞凋亡是程序性细胞死亡过程，在机体的发生发展及自身稳定中起着关键作用，对肿瘤细胞的生长具有抑制和免疫监视作用。肿瘤坏死因子相关的凋亡诱导配体（TNF-related apoptosis-inducing ligand，TRAIL）是TNF超家族的新成员，它与其特异性死亡受体结合，激活多种凋亡信号传导途径，诱导细胞凋亡［1］。赵湜等［2］检测了TRAIL及其受体在急性髓系白血病细胞中的表达，发现患者组TRAIL、DR4和DR5表达高。然而，关于化疗药