哮喘大鼠血管内皮生长因子表达与γ干扰素、白介素4变化的相关性及地塞米松的干预_临床医学论文.docVIP

哮喘大鼠血管内皮生长因子表达与γ干扰素、白介素变化的相关性及地塞米松的干预_临床医学论文 哮喘大鼠血管内皮生长因子表达与γ干扰素、白介素变化的相关性及地塞米松的干预_临床医学论文 【摘要】 观察血管内皮生长因子(vascular endothelial growth factor，VEGF)、γ干扰素（IFN）、白介素（IL）在大鼠哮喘急性模型中的表达情况，探讨Th细胞亚群的失衡与其表达的相关性。方法：36只清洁级雄性SD大鼠随机分为生理盐水对照组（C组）、哮喘组（A组）和地塞米松干预组（D组），以卵清白蛋白(ovalbumin，OVA)致敏激发法制备大鼠哮喘急性模型，末次激发24 h后腹腔注射麻醉，心脏取血，右肺行支气管肺泡灌洗留取灌洗液(BALF)。应用酶联免疫吸附(ELISA)法测定血清和BALF中IL、IFN和VEGF的浓度。结果：A组血清和BALF中IL、VEGF的水平均分别显著高于C组，D组血清和BALF中IL、VEGF的水平均分别显著低于A组（0.01），C组和D组相比差异均无统计学意义（Pgt;0.05）。A组血清和BALF中IFN的水平均显著低于C组（0.01），D组血清、BALF中的IFN水平均显著高于A组（0.01）而低于C组（0.01）。大鼠BALF中IL浓度与VEGF浓度呈显著正相关(r= 0.797， 0.01)，IFN浓度与VEGF浓度呈显著负相关(r=-0.568， 0.01)。大鼠血清中IL浓度与VEGF浓度呈显著正相关(r=0.707， 0.01)，IFN浓度与VEGF浓度呈负相关(r=-0.332，0.05)。结论：大鼠哮喘急性模型VEGF蛋白表达显著增高，Th1细胞因子IFN的表达与VEGF的表达存在负相关，Th2细胞因子IL的表达与VEGF的表达存在正相关。地塞米松可抑制大鼠哮喘急性模型中VEGF和IL的表达，可能参与抑制哮喘气道炎症和重塑的重要机制之一。 【关键词】 哮喘 血管内皮生长因子 干扰素 白介素 地塞米松 ［Abstract］ Objective：To study the relationship between imbalance of Th1/Th2 cytokines and expression of VEGF, IFNSD) rats were randomLy divided into three groups on average, including asthma group (group A), control group (group C) and dexamethasonetreated group (group D). In this experiment, the rat model of asthma was established by the ovalbumin (OVA) provocation methods. 24 hours after the last provocation, the animals were sacrificed. Blood and bronchoalveolar lavage fluid (BALF) were collected. The concentrations of IL4, IFNγ and VEGF in serum and BALF were measured by enzymelinked immunosorbent assay (ELISA). Results: The concentrations of IL4、VEGF in serum and BALF in group A ［(38.56±5.09) pg/mL, (22.62±4.00) pg/mL, respectively ＆ (381.81±45.45) pg/mL, (34.58±3.07) pg/mL］ were significantly higher than those in group C ［(14.39±1.13) pg/mL, (10.49±1.50) pg/mL, respectively］(0.01), ［(285.36±53.75) pg/mL, (22.88±3.18) pg/mL, respectively］(0.01); and the concentrations of IL、VEGF in serum and BALF in group D ［(14.98±2.48)pg/mL, (10.68±1.13)pg/mL, respectively］［(247.75±47.81)pg/mL, (22.80±2.93)pg/mL, respectively］were significan