增生性玻璃体视网膜病变中组织型转谷氨酰胺酶的表达_临床医学论文.docVIP

增生性玻璃体视网膜病变中组织型转谷氨酰胺酶的表达_临床医学论文 增生性玻璃体视网膜病变中组织型转谷氨酰胺酶的表达_临床医学论文 作者：王辉　刘哲丽　郝旭红　徐丽 【摘要】 　　目的: 检测增生性玻璃体视网膜病变（PVR）视网膜前膜和视网膜色素上皮（RPE）细胞中组织型转谷氨酰胺酶(tTG)的表达情况，探讨tTG是否参与PVR的发病过程。方法: PVR患者21例,收集视网膜前膜，C级8例，D级13例, 行tTG免疫组织化学染色。另对培养3～5代的人RPE细胞分别用含有100mL/L PVR玻璃体液、正常玻璃体液和PBS的DMEM培养液进行孵育24h后行tTG免疫细胞化学染色。光镜下观察，比较C级和D级膜的表达阳性率，显微图像分析测量3组RPE细胞染色的平均灰度值。结果：在PVR视网膜前膜中tTG呈阳性表达（81％）， C级和D级膜表达阳性率无差异（C级88％，D级77％，P gt;0.05）。培养的人RPE细胞表达tTG，在PVR患者的玻璃体液作用下，tTG表达的平均灰度值为137.0±2.6，与正常玻璃体液组（143.5±2.9）及PBS对照组（143.6±3.0）相比，表达水平升高（P lt;0.05）。结论：PVR视网膜前膜和培养的人RPE细胞tTG表达阳性，在PVR患者的玻璃体液作用下，RPE细胞的tTG表达水平升高，这表明tTG参与了PVR的发病过程，在PVR视网膜前膜的形成过程中可能有重要作用。 【关键词】 增生性玻璃体视网膜病变　组织型转谷氨酰胺酶　视网膜色素上皮细胞 　　Tissue transglutaminase in proliferative vitreoretinopathy Abstract AIM: To investigate the expression of tissue transglutaminase (tTG) in proliferative vitreoretinopathy (PVR) epiretinal membranes and retinal pigment epithelium (RPE) cells which is thought to be a key element in PVR formation. METHODS: Twenty-one samples of epiretinal membranes were obtained from patients with PVR involved previous rhegmatogenous retinal detachment. Eight samples were at C stage of PVR, 13 samples were at D stage. The samples were selected to perform immunohistochemical staining. Third- to fifth-passage human RPE cells were incubated by serum-free DMEM containing 100mL/L PVR vitreous samples, normal vitreous samples or PBS and stained immunocytochemically. Positive rates of expression were calculated in both C and D stage membranes under light microscopy. The staining average gray values of the three group RPE cells were detected with image analyzing system. RESULTS: The expression of tTG was present in PVR epiretinal membranes (80%). Positive staining was most prominent in ECM, fibroblast-like cells and macrophages. There was no significant difference in positive rates between C and D stage membranes (P gt;0.05). Cultured human RPE cells expressed tTG, and the expression level of RPE cells incubated with PVR vitreous samples was higher th