大鼠膝关节不稳定性OA模型的软骨及软骨下骨改变_临床医学论文.docVIP

大鼠膝关节不稳定性OA模型的软骨及软骨下骨改变_临床医学论文 大鼠膝关节不稳定性OA模型的软骨及软骨下骨改变_临床医学论文 作者：杨勇，吴世栋，张小钰，金群华 【摘要】 ：目的 通过建立大鼠膝关节前交叉韧带切断骨性关节炎（OA）动物模型，探讨该模型中软骨及软骨下骨病理变化。方法 取SD大鼠30只随机分成３组（术后2、6周和10周组）。每只大鼠右膝行前交叉韧带切断，造成膝关节不稳定动物模型，对侧为假手术侧。分别将每组动物于术后2、6和10周处死，取胫骨近段，比较各组骨关节大体形态及病理变化。光镜下观察指标有改良的Ｍankin评分、骨组织形态计量学参数。免疫组化法观察MMP-13在软骨细胞中的表达。结果 在造模后2、6周和10周软骨退变呈进行性发展。Ｍankin评分增高、MMP-13表达逐渐增强，与假手术侧相比有统计学意义（P＜0.01）。软骨下骨形态计量学分析显示，造模后早期软骨下骨骨量轻度减低（P＜0.05），之后骨量逐渐增加（P＜0.01）。结论 大鼠的前交叉韧带切断能够造成动物膝关节OA样改变，表现出软骨下骨吸收、骨重建的变化特点。 【关键词】 骨性关节炎；动物模型；软骨下骨；基质金属蛋白酶13 　　Abstract：Objective To establish an osteoarthritis model in rat knees by transacting anterior cruciate ligament，and characterizing subchondral bone remodeling， cartilage damage during the disease progression in the models of surgically induced OA. Methods 30 rats were divided into three group(2weeks、6weeks and 10weeks post-surgery group). Rats right knee joints were subjected to anterior cruciate ligament transection (ACLT)，while the left knee served as control. Rats were sacrificed and the tibil plates were harvested which were studied in both gross-morphological and pathohistological ，at the same time Coimmunostaining for matrix metalloproteinase13 (MMP-13) was performed to investigate the progression of cartilage degradetion. Results The degeneration of articular cartilage of the surgery knees got worse by time. surgery knees Mankins scores and MMP-13 expressions were significantly higher than those in the sham knees (P＜0.01). Histomorphometric analysis revealed subchondral bone loss within 2 weeks postsurgery(P＜0.05)followed by significant increases in subchondral bone volume relative to sham up to 10 weeks post-surgery(P＜0.01). Conclusion WACLT on rats can induce Osteoarthritic changes which involve cartilage and subcondral bone. This model have the merits of economical，convenient and short-terming. 　　Key words：osteoarthritis OA; animal model; subcondral bone; matrix metalloproteinase13 　　动物模型是研究骨性关节炎（osteoarthritis，ＯＡ）病理机制、治疗及预防的重要手段之一。目前OA的动物造模方法有多种，目的是最大程度地模