大鼠门脉高压症脾次全切除术对部分免疫功能的影响_临床医学论文.docVIP

大鼠门脉高压症脾次全切除术对部分免疫功能的影响_临床医学论文 大鼠门脉高压症脾次全切除术对部分免疫功能的影响_临床医学论文 作者：刘程伟 张雪松 王树卿 罗维 张强 【摘要】目的：通过门脉高压症（PHT）脾次全切除术后细胞免疫和体液免疫的变化，初步评价脾次全切除术在门脉高压症中的作用。方法：建立肝硬化PHT大鼠疾病模型。实验分5组，每组10只。正常对照组（NCG），实验对照组（ECG），全脾切除组（TSG），保留正常脾脏50％的脾部分切除组（R50％NSG），保留正常脾脏大小的脾部分切除组（RNSG）。术后4周对各组进行血常规、免疫球蛋白、T淋巴细胞亚群的测定及病理学检查。结果：术后4周R50％NSG组与RNSG组与NCG组和ECG组血常规、免疫球蛋白和T淋巴细胞亚群水平差异无统计学意义。ECG光镜下见假小叶，再生的肝细胞结节肝细胞索排列紊乱等肝硬化表现脾包膜增厚，脾实质充血严重。脾内纤维严重增生。R50％NSG和RNSG光镜下见肝纤维化程度减轻，脾包膜变薄，脾实质轻度充血。结论：PHT残余脾脏具有一定的生理功能；保留部分脾脏的脾部分切除术在一定程度上能够维持机体的体液免疫和细胞免疫。 【关键词】脾次全切除术#12539;门静脉高压症，门静脉#12539;免疫功能 The influence of subtotal splenectomy on partial immunlogical function in rats with poroal hypertension 【ABSTRACT】Objective:To detect the change of cell immunity and body fluid immunity after subtotal splenectomy in portal hypertension of rat ,and preliminarily evaluate the role of subtotal splenectomy in portal hypertension.Methods:We established the rat model of hepatic cirrhosis portal hypertension.Rats were divided to five groups:normal control group(NCG);experimental control group(ECG),total splenectomy group(TSG),retain fifty percent size of normal spleen group(R50%NSG),retain the same size of normal spleen group(RNSG).Measured the Blood routine,Immunoglobulin,T Iymphocyte subgroup and stained with hematoxylin and eosin (HE) at 4 weeks after surgery.Results:There were not significant different between RNSG and NCG in Blood routine,Immunoglobulin,T Iymphocyte subgroup at 4 weeks after operation.ECGcould be seen by light microscope that the tissue of normal hepatic lobule was destructed,and appeared pseudolobule.Disarrangement of regenerated hepatic cell nodus and liver cell cord,fatty degeneration in hepatic cell,thickening of spleen capsule and serious congestion of spleen parenchyma,atrophy of white pulp and shrinking of follicle and germinal center R50%NSG and RNSG could be seen that hepatic fibrosis,spleen capsule thichening and congesition of splenic parenchyma to a less extent.Conclusion:Remaining spleen of portal hyperte