体重监测下硫代乙酰胺诱导豚鼠肝纤维化模型_临床医学论文.docVIP

体重监测下硫代乙酰胺诱导豚鼠肝纤维化模型_临床医学论文 体重监测下硫代乙酰胺诱导豚鼠肝纤维化模型_临床医学论文 作者：王小平，赖远辉，许峰峰，甄宇洋 【摘要】 目的 根据体重变化监测硫代乙酰胺(TAA)诱导豚鼠肝纤维化过程中个体反应差异,探索硫代乙酰胺诱导豚鼠肝纤维化模型的可行性。方法 健康雄性豚鼠50只，随机分成三组：对照组10只;实验组40只(TAA六周组20只，TAA九周组20只)。实验组予浓度为0.03%TAA+750 mg/L Vit C饮用水诱导并不限量供应。肝组织用HE染色，Masson三色染色、Gomori银染色法。据肝组织内纤维组织增生状况分为5级：(S0，S1，S2，S3，S4);统计各个体肝纤维化程度。结果 体重比较使用TAA第七周：TAA六周组vs. TAA九周组，Pgt;0.05，实验组间差异无统计学意义。各实验组内个体体重变化存在差异。纤维化形成统计：S0：(对照组10例)，S1：TAA六周组4例，S2:TAA六周组14例，TAA九周组5例，S3:TAA六周组1例，TAA九周组9例，S4:TAA九周组2例。结论 本实验表明硫代乙酰胺可以诱导豚鼠肝纤维化模型。体重监测是一种无创、简便、安全、有效的监测手段。 【关键词】 肝纤维化;硫代乙酰胺;豚鼠 　　[Abstract] Objective To observe guinea pig’s hepatic and systemic responses to thioacetamide (TAA) during induction of hepatic fibrosis were monitored by weekly-weight changes,to explore the feasibility of inducing hepatic fibrosis model in guinea pigs.Methods Fifty healthy male guinea pigs were divided randomly into three groups:control group(n=10); experimental group(n=40):the six-weeks group(n=20) and the nine-weeks group(n=20) received continuous administration of 0.03% (w/v) TAA+ 0.75% Vit-C in the drinking water.Control group (n=10) received the same concentration of 0.75% Vit-C in the drinking water.The body weights were measured weekly once at same time.Sections of liver about 5 μm thickness were stained with HE and Masson trichrome and Gomori staining method.Fibrosis was classified as:S0，S1，S2，S3，S4.Statistics of all individuals in each group in the degree of hepatic fibrosis were done.Results After in-taking TAA,there was non-significant difference between experimental groups weekly,Pgt;0.05.Each individual’s body-weight has significant difference in experimental groups.S0:control group were 10 cases;S1:TAA six-weeks group 4 cases;S2:TAA six-weeks group 14 cases,TAA nine-weeks group 5 cases;S3:TAA six-weeks group 1 cases,TAA nine-weeks group 9 cases;S4:TAA nine-weeks group 2 cases.Conclusion This experiment showed that thioacetamide can induce hepatic fibrosis model in guinea pigs; Weight monitoring is a non-invasive,s