宫颈上皮内瘤变和宫颈癌组织中Survivin与Bcl-2的表达及相关性_临床医学论文.docVIP

宫颈上皮内瘤变和宫颈癌组织中Survivin与Bcl-2的表达及相关性_临床医学论文 宫颈上皮内瘤变和宫颈癌组织中Survivin与Bcl-2的表达及相关性_临床医学论文 作者：朱华，施铮铮，张虎祥，万丽，杨孝军，郑飞云 【摘要】 　　目的 ：研究Survivin、Bcl-2在宫颈上皮内瘤变及宫颈癌中的表达，探讨两者在宫颈癌发病机制中的作用。方法：采用原位杂交法检测宫颈上皮内瘤变（CIN）和宫颈癌中Survivin基因的表达，以正常宫颈组织为对照。同时采用免疫组织化学法检测Bcl-2蛋白在各组中的表达。结果 ：Survivin mRNA的阳性率在宫颈癌、CIN和正常宫颈组织中分别为77.8%、44.6%和0%，Bcl-2蛋白在三者中的阳性表达率分别为70.4%、39.2%和0，两者差异均有显著性。Survivin及Bcl-2阳性表达在CINⅢ级中明显高于CIN I/Ⅱ级，在宫颈癌Ⅱb～Ⅲ期中显著高于Ⅰ～Ⅱa期，在低分化组中显著高于高、中分化组。在淋巴结转移组中Survivin的表达显著高于无淋巴结转移组。在宫颈癌中Survivin与Bcl-2的表达呈正相关。结论：Survivin和Bcl-2的异常表达可能参与宫颈癌的发生、发展过程，可能有协同促进抗凋亡作用，并提示与肿瘤的恶性程度和不良预后有关。 【关键词】 Survivin Bcl-2 宫颈癌 CIN 　　Abstract: Objective: To investigate the expression of Survivin and Bcl-2 in cervical intraepithelial neoplasia and cervical carcinoma， and to explore the role of them in cervical carcinoma pathogenesis. Methods: The expression of Survivin mRNA was detected with hybridiza-tion in situ in CIN and cervical carcionma，and the expression of Bcl-2 was detected with immunohistochemical method. Normal cervical tissues were acted as the control. Results: The positive rates of Survivin mRNA in cervical carcinoma，CIN and the normal cervical tissues were 77.8%，44.6% and 0%，respectively；while the expression of Bcl-2 was 70.4%，39.2% and 0% in the above three groups. There was significant difference among those groups. The expression of Survivin and Bcl-2 in CINⅢ was obviously more significant than those in CIN I/Ⅱ. And the differences were obviously more significant in cervical carcinoma at stage Ⅱb～Ⅲ than those at stageⅠ～Ⅱa. The expressions of Survivin and Bcl-2 with poorly differentiation were also obviously more significantly different than those with good or moderated differentiation. The expression of Survivin in cervical carcinoma with lymphatic metastasis was more significant difference than those without lymphatic metastasis. In our study，positive correlation between Survivin mRNA and Bcl-2 was observed in cervical carcinoma. Conclusion: The abnormal expression of Survivin and Bcl-2 may particip