重复热应激致公兔性腺氧化损伤地研究.pdfVIP

重复热应激致公兔性腺氧化损伤的研究 杨淑华1,2何剑斌1于立辉1 李林1徐世文2 1.沈阳农业大学 畜牧兽医学院，辽宁沈阳110866 2.东北农业大学 动物医学院，黑龙江 哈尔滨150030 摘 要：为探讨热应激对雄性兔性腺的氧化损伤机制，以110日龄、体重相近的雄性新西兰兔为研究对象，每天在固定时 间分别以31℃-34℃和38℃-40℃进行热应激，分别在热应激后15d、30d、45d及60d处死取样，检测性腺组织内总抗氧化 酶(T-AOC)活性、丙二醛(MDA)及蛋白质羰基含量。结果显示，随温度升高，睾丸和附睾组织内MDA含量增加，T-AOC 酶活性降低，蛋白质羰基含量呈上升趋势，表现出明显的温度-时间效应。表明高热可改变兔睾丸和附睾正常的生理机能， 使自由基在组织内累积，引起蛋白质氧化损伤，进而降低繁殖机能。 关键词：雄性新西兰兔；氧化损伤；热应激；性腺 Effectofreduplicativeheatstressonoxidationdamageinmalerabbitsgonad YANGShuhua12HEJianbin2YULihui2LIlin2XUShiwen1 1.DepartmentofVeterinaryMedicine,NortheastAgriculturalUniversity,HeiLongjiangHarbin150030,China 2.DepartmentofAnimalHusbandryandVeterinaryMedicine,ShenyangAgriculturalUniversity,LiaoNingShenyang110866, China Abstract:Todeterminetheeffectofheatstressonoxidativedamageinmalerabbitsgonad,110daysNew Zealandrabbitswiththesimilarweightwereusedinthisstudy.Theserabbitsweresufferedheatstressat31℃ -34℃and38℃-40℃ everydayinfixedtime,thesamplesweretakenaftertheheatstressat15d,30d,45dand 60drespectively,thenthemalondialdehyde(MDA),totalantioxidantcapacity(T-AOC)andproteinoxidative statusintestisandepididymisweredetermined.Theresultsindicatedthatwithtemperatureincrement,theMDA contentoftestisandepididymisincreased,theT-AOCactivitydecreasedandtheproteincarbonylcontenthadthe risingtrend,anddisplayssignificanttemperature-timeeffects.Thisresearchindicatedthattheheatstressmay changetherabbittesticleandtheepididymisnormalphysiologyfunction,makesthefreeradicalaccumulateinthe organization,causestheproteinoxidationdamage,reducesthereproductionfunction Keywords:NewZealandmalerabbits;oxidationdamage;heatstress;gonad 试验数据边 j质羰基鲁 刍势。低壹 平衡，促侣 量活性氧雨