糖尿病大鼠视网膜中IGF-1 的动态表达及巴曲酶对其的影响.docVIP

论 著 Original Article The Dynamic Expression of IGF-1 and the Impact of Batroxobin on it in the Retina of Diabetic Rats 王宇石 程门雪 饶明俐 WANG Yushi CHENG Menxue RAO Mingli 吉林大学第一医院（中国132011） The First Hospital of Jilin University (China 132011) 中图分类号：587.1 文献标识码：A 文章编号：1818－0086（2008）01 摘 要：目的 探讨胰岛素样生长因子（IGF-1)在糖尿病大鼠视网膜中的动态变化规律，初步证实巴曲酶对IGF-1的影响并探讨其机制，为临床上治疗糖尿病视网膜病变（DR）提供理论依据。 将大鼠腹腔注射链脲佐菌素制作实验性糖尿病模型，再分别于成模后2个月和3个月时腹腔注射巴曲酶进行干预。通过免疫组织化学和原位杂交双重检测IGF-1在视网膜中的表达。 DM造模后2个月时视网膜中IGF-1的含量明显增加，且随时间变化有统计学差异，而巴曲酶治疗后IGF-1的表达明显增加。 DR大鼠视网膜中IGF-1表达增加可能参与DR的发病机制。巴曲酶对DR有保护作用，其机制可能包括对IGF-1的调节。 ：糖尿病视网膜病变大鼠；胰岛素样生长因子；巴曲酶 To investigate the dynamic regularity of IGF-1 in the retina of diabetic rats and to confirm the impact of Batroxobin on IGF-1 tentatively and the mechanism in order to furnish the theory foundation for the therapy of DR clinically. Method Through the peritoneal injection of streptozocin to make the experimental diabetes models, we injected Batroxobin into abdominal cavity of rats differently 2 months and 3 months after the formation of DR models for intervention. Through the immunohistochemistry and hybridization in situ to detect the expression of IGF-1 in the retina of the DR rats. Result The content of IGF-1 in the retina of the DR rats decreased obviously 2 months after the formation of DM models and it had time-varying statistics diversity, however the expression of IGF-1 was markedly increased after the treatment of Batroxobin. Conclusion The decreased expression of IGF-1 in the retina of DR rats might play an important role in the pathogenesis of DR. Treatment with Batroxobin is beneficial in the prevention of DR，possibly through the regulation of the expression level of IGF-1 in the retina of the DR rats. Key words: DR rats；IGF-1；Batroxobin 糖尿病视网膜病变(diabetic retinopathy，DR)作为DM主要并发症之一，是人类最主要的致盲因素，在各型DM患者中DR的发生率为24%～70%[1]。DR的发病机制尚未清楚，但起因是视网膜异常新生血管的形成[2]。有人推测，胰岛素样生长因子（IGF-1）可能参与DR的发生[3]。巴曲酶是从中南美产的蛇毒精制提炼的类似凝血酶作用的一种酶，具有抗凝、降低血液粘稠度、改善微循环的药理作用，目前已广泛应用于缺血性