HLA—DRB多态性与HBV感染的相关性研究.pdfVIP

63 · 临床论著 · HLA—DRB多态性与HBV感染的相关性研究 邢文斌 郭晓楠 徐慧宁 【摘要】目的 通过对慢性乙型肝炎男性患者的配偶和子女HBV感染状况及其HLA．DRB多态 性分析，揭示HLA—DRB与HBV感染的相关性。方法 采用ELISA方法筛查HBV血清标志物，采 用PcR／SSP技术扩增HBV感染者及易感人群HLA．DRB1不同位点等位基因片段，琼脂凝胶 电泳分 离基 因片段，紫外透射反射分析仪判定HLA—DRB1型别。结果 慢性乙型肝炎患者及 HBV携带者 携带HLA-DRB10201、0301基因显著高于健康对照组；HLA—DRB11301在健康对照组的携带率显 著高于慢性乙型肝炎患者及病毒携带者；HLA．DRB10201、0301、0701和 1301在感染后恢复组与 健康对照组相比携带率差异无统计学意义；HLA—DRB10201、0301、0701和 1301等位基因在肝炎 组与携带组中差异无统计学意义；HLA．DRB10201、0301和 0701在肝炎组携带率率较高，HLA— DRB11301在感染后恢复组携带率较高。结论 HLA—DRB10701、0301、0201与HBV慢性感染有关， HLA．DRB11301与HBV的清除有关。 【关键词】HBV感染；肝炎，乙型，慢性；配偶和孩子；HLA—DRB1；等位基因 ThecorrelationbetweenHLA。DRB polymorphism andHBV infeorion XING Wenb吮 GUOXiaonan，XU Huining．TheDepartmentoflnfectiousDiseases, PSixthPeoplesHospitalofDalian．Dalian116031．China Correspondingauthor：XINGWenbin，Email：xingwb123@126．com [Abstract] ObjectiveToanalyzetheHBV infectionstatusandtheirHLA-DRBpolymorphism amongmalepatientswithchronichepatitisB，meanwhiletheirspousesandchildrenwerecollected，to ]dent]fythecorrelationbetweenHLA-DRB andHBVoM ethods Theselum markersofhepatitisB were testedbyELISA．differentHLA—DRB1allelicfragmentsinthepatientswithHBV andtheirspousesand childrenwereamplifiedbyamplification，andthegenefragmentswereseparatedbyagargelelectrophoresis， UV transmittancereflectanceanalyzerdeterminethetypeofHLA—DRB1．Results Thepatientswith chronichepatitisB andHBV carrierscarryingHLA—DRB10201．0301genesweresignificantlyhigherthan thoseofhealthycontrolgroup。TherfequencyofHLA—DRB1 1301alleleinhealthycontrolgroupwere higherthanthoseinHBV patientsandcarriers．Therewasnosignificantdifferencebetweentherecovery groupandhealthycontrolgroupwithHLA—DRB10201，0301，0701and1301alleles．Therfequencyof HLA—DRB10201，0301，0701and 1301allelesbetweenthepatientswithchronichepatitisB andHBV carrierswerenosignificantdifference．TherfequencyofHLA—DRBl0201．0301and0701allelesinthe hepatitisgroupwashigher,andHLA—DRB1 1301alleleinrecoverygroupwashigher．ConclusionsHLA— DRB10701，0301and0201alleleswe