不同诱导子对黄芪悬浮培养细胞中黄芪多糖积累的影响.pdfVIP

安徽农业科学．JournalofAnhuiAgIi．Sci．2014。42(26)：8954—8956，8959 责任编辑 黄小燕 责任校对 况玲玲 不同诱导子对黄芪悬浮培养细胞中黄芪多糖积累的影响 杨静，剥、皓 (天津渤海职业技术学院，天津300402) 摘要 [目的]研究水杨酸、茉莉酸甲酯、硝酸银、高糖、高盐、超声等诱导子对黄芪细胞生长和黄芪多糖合成的影响。[方法]向细胞悬浮 液中加入各种诱导子或对细胞悬浮液进行超声处理，测定细胞干重增殖倍数和黄芪多糖的合成量。[结果]加入 10．0ms／L水杨酸可使 黄芪多糖的含量为对照组的2．o4倍 ；添加较低浓度的茉莉酸甲酯浓度 (1．0ms／L)使黄芪多糖的含量达48．974ms／g，比对照提高了 2．50倍；添加4mg／L浓度的硝酸银能使黄芪多糖含量提高1．6o倍；高糖和高盐胁迫处理均能诱导黄芪多糖的合成。高糖处理能使黄芪 多糖的产量比对照提高了1．92倍。低剂量的超声刺激对黄芪细胞生物量的积累有显著的刺激作用。[结论]诱导子的添加可有效促进 黄芪悬浮培养细胞中黄芪多糖的积累。 关键词 诱导子；黄芪多糖；积累；细胞悬浮培养；黄芪 中图分类号 S567 文献标识码 A 文章编号 05l7—6611(2014)26—08954—03 EffectofDifferentElicitorsonAccumulationofAstragalusPolysaccharidesintheSuspensionCultureCellofAsWagalusmembrana- Cel YANGJjngetaI (TianjinBohaiVocationalandTechnicalCollege，Tianjin300402) Abstract [Objective]Toinvestigatetheeffectsofdifferentelicitors(salicylicacid，methyljasmonate，silvernitrate，highsucrose，hig【h salt，ulrtasonicstimulation)onhtecellgrowthandinducibleformationofastragaluspolysaceharidesinAstragalusmembranac~ll$cens．[Meth一 0d]Thesuspensionculturecellsweretreatedwiht hteelicitorsorultrasonicstimulation．Thenthecellgrowthrateandhteasrtagaluspolysac． ehafidescontentinAstragalusmembranaceucellsweredetermined．[Result]10．0mg／Lsalicylicacidresultedinna increaseofastragalus polysaccharidescontentbyabout2．04times．Withhteadditionofmehtyljasmonate，htehighestcontentofastragaluspolysacchafidesreached 48．974mg／g．anditwas2．50timeshigherthna hteeonto1． eadditionof4mg／Lsilvernitratepromoteastragaluspolysaccharidescontent by1．60times．Bothhtehighsucroseandhihg salttreatmentcouldinducethesynthesisofastragaluspolysaceharides．Wihtthehighsucrose treatment。theastragatuspolysaeehafidescontentwasincreasedby1．92times．Low does ofultrasonicstimulationcansignificantlystimulate thecellgrowth．[Conclusion] eadditionoftheelicitorswaseffectivetoenhancehtefomrationofasrtagaluspolysaccharidesinAstragalus membranaceuscells． Keywords Elicitors；Astragaluspolysaeehafides；Acc