二维相关近红外光谱在快速检测掺伪牛奶中应用.pdfVIP

二维相关近红外光谱在快速检测掺伪牛奶中的应用 杨仁杰1,2刘蓉1陈文亮1徐可欣1 1.天津大学精密测试技术及仪器国家重点实验室，天津300072 2.天津农学院机电工程系，天津300384 摘要：为了检验牛奶是否掺杂及对掺杂物量化，配置了掺有不同含量的尿素和三聚氰胺牛奶样品各40个。首先研究了纯 牛奶与掺杂牛奶的常规二维近红外光谱特性，然后以牛奶中掺杂目标物浓度为外扰，构建二维相关谱 （近红外-近红外，近 红外-中红外），研究了掺杂牛奶的二维相关谱特性。最后，在牛奶二维相关谱特性的基础上，将二维相关近红外谱矩阵与化 学计量学结合起来，分别采用展开偏最小二乘法(unfoldPLS)和多维偏最小二乘法(NPLS)建立牛奶中掺杂物的定量分析 模型。研究结果表明：NPLS模型比unfoldPLS模型具有更好的预测能力，该模型对尿素和三聚氰胺的预测均方根误差 (RMSEP)分别为0.134g/L、0.09g/L。 二维相关近红外谱；多维偏最小二乘法;掺杂牛奶;尿素；三聚氰胺 ApplicationofTwo-dimensional(2D)Near-infraredCorrelation SpectroscopyinRapidDetectionofAdulterantinMilk YANGRen-jie1,2 LIURong1ChenWenliang1XUKe-xin1 1.Statekeylaboratoryofprecisionmeasuringtechnologyandinstruments,TianjinUniversity,Tianjin300072,China 2.DepartmentofElectromechanicalEngineering,TianjinAgriculturalUniversity,Tianjin300384,China Abstract:Forthedetectionandquantificationofadulterantinmilk,40adulteratedmilksampleswithaddeddifferentcontentofurea andmelaminewerepreparedindividually.Thecharacteristicsofpuremilkandadulteratedmilkwerestudiedbyone-dimensional spectra.Then2DNIRand2DNIR-IRcorrelationspectroscopywerecalculatedundertheperturbationofadulterationconcentration. Atthesametime,thecharacteristicsof2Dcorrelationspectroscopywerestudied.Basedonthecharacteristicsof2Dcorrelation spectroscopy,boththeunfold-PLSandNPLSmodelswerebuiltusing2Dnear-infraredcorrelationspectroscopymatrixindividually. TheresultsindicatedNPLSmodelwasmorerobustforaccuratequantificationoftheconcentrationofadulterantinmilkthanunfold PLSmodel.IncaseofNPLStherootmeansquareerrorsofprediction(RMSEP)were0.134g/Land0.09g/Lrespectively. Key words: Two-dimensional correlation near-infrarod spectroscopy;Multi-way partial least squares;Adulterated milk;Urea;Melamine 得分向{ 晌增大， m-I区 间 钓被覆盖 了一个掂1 1动所引i f；+l.Ol~ etal.Rar )-67. ispectros00py ：品安全检测{ 巳织光学与