LAMP技术在鱼类疾病诊断中的应用.pdfVIP

Parasitol Res (2010) 106:1379– 1383 DOI 10.1007/s00436-010-1812-3 ORIGINAL PAPER Sensitive and rapid detection of Clonorchis sinensis infection in fish by loop-mediated isothermal amplification (LAMP) X. Q. Cai M. J. Xu Y. H. Wang D. Y. Qiu G. X. Liu A. Lin J. D. Tang R. L. Zhang X. Q. Zhu Received: 8 February 2010 /Accepted: 19 February 2010 /Published online: 16 March 2010 # Springer-Verlag 2010 Abstract The fish-borne clonorchiasis caused by the oriental from the C. sinensis cathepsins B3 gene were evaluated, liver fluke Clonorchis sinensis is endemic in a number of and specific amplification products were obtained with countries with over 35 million people being infected C. sinensis , while no amplification products were detected globally. Rapid and accurate detection of C. sinensis in its with DNA of related trematodes, demonstrating the specificity intermediate host fish is important for the control and of the assay. The LAMP assay was proved to be 100 times prevention of clonorchiasis in areas where the disease is more sensitive than a conventional polymerase chain reaction endemic. In the present study, we established a loop- for detection of C. sinensis. The established LAMP assay mediated isothermal amplification (LAMP) approach for provides a useful tool for the rapid and sensitive detection of the sensitive and rapid detection of C. sinensis metacercariae C. sinensis in fish, which has important implications for the in fish. The specificity and sensitivity of primers designed effective control of human clonorchiasis. Introduction X. Q. Cai and M. J. Xu contributed equally to this work. The