抗HCMV人源化噬菌体抗体库的构建.pdfVIP

第 26 卷第 14 期 第 　三 　军 　医 　大 　学 　学 　报 Vol. 26 , No. 14 　　　　 　　　　 2004 年 7 月 ACTA 　ACADEMIAE 　MEDICINAE 　MILITARIS 　TERTIAE Jul. 2004 1239 ( ) 文章编号 2004　 论著 抗 HCMV 人源化噬菌体抗体库的构建 (第三军医大学大坪医院野战外科研究所第一研究室 ,创伤、烧伤与复合伤国家重点实验室 ,重庆 400042) 顾长国 ,刘　琛 ,李　磊　　 　　提 　要 : 目的 　构建抗巨细胞病毒噬菌体抗体库 ,为制备巨细胞病毒感染治疗用单克隆抗体奠定基础。方法 　以巨 细胞病毒感染患者外周血淋巴细胞为材料 ,建立噬菌体抗体库 ,并对其进行鉴定。结果 　构建完成了一个库容为 6. 5 × 6 10 cfuml 的噬菌体抗体库。结论 　人源化抗巨细胞病毒噬菌体抗体库的建立为下一步抗体的筛选和表达奠定了基础。 　　关键词 : 巨细胞病毒;噬菌体抗体库 ;构建 　　中图法分类号 : R373. 9 ; R392. 11 ; R3943 文献标识码 : A Construction of humanized phage display antibody library for human cytomegalovirus GU Changguo , LIU Chen , LI lei ( State Key Laboratory of Trauma , Burn and Combined Injury , Department 1 , Institute of Surgery Research , ) Daping Hospital , Third Military Medical University , Chongqing 400042 , China 　　Abstract : Objective 　To construct a phage display antibody library of human cytomegalovirus ( HCMV) for providing the basis of screening humanized antibody against HCMV infection. Methods 　The peripheral lymphocytes from HCMVinfected patients were collected and the phage display antibody library were constructed and identified. 6 Re sults 　A phage display antibody library with titer of 6. 5 ×10 cfuml has been constructed. Conclusion 　The li brary has laid the foundation for further screening and expression of the specific antibody in the future. 　　Key words : human cytomegalovirus ; phage display antibody library ;