阪崎肠杆菌_克罗诺杆菌属_分子检测方法研究进展.pdfVIP

350 2011, Vol. 32, No. 13 食品科学 ※专题论述 阪崎肠杆菌(克罗诺杆菌属)分子检测方法 研究进展 1 1,2 1, 1 王 翔 ，祝长青 ，徐幸莲 * ，周光宏 ( 1.南京农业大学教育部肉品加工与质量控制重点实验室，江苏 南京 2 10095 ； 2.江苏出入境检验检疫局，江苏南京 2 1000 1) 摘 要：阪崎肠杆菌(现克罗诺杆菌属)是一种重要的食源性条件致病菌，因其能通过污染婴幼儿配方食品导致严重 的新生儿脑膜炎、菌血症和小肠结肠炎等疾病，而受到广泛的关注。其分子生物学检测方法克服了传统检测方法 实验操作繁琐、耗时长等问题。目前常用的分子生物学方法主要有普通P CR 、实时荧光定量P C R 、环介导恒温 扩增、探针方法等。 关键词：阪崎肠杆菌( 克罗诺杆菌属) ；分子检测方法；环介导恒温扩增；分子探针 Recent Advances in Studies on Molecular Detection Methods for Enterobacter sakazakii 1 1,2 1, 1 WAN G Xiang ，ZHU Ch ang-qin g ，XU Xing-lian * ，ZHOU Guang-hon g ( 1. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanj ing 2 10095, China；2. Jiangsu Entry Exit In spection and Quarantine Bureau, Nanjing 2 1000 1, China) Abstract ：Enterobacter sakazakii (Cronobacter spp .) is an emerging food-borne conditional pathogen associated with meningitis, bacteremia and necrotizing enterocolitis; especially neonatal meningitis is of high potential danger in neonates. Many bio-molecular assays like regular PCR, real-Time PCR, loop-mediated isothermal amplification , and molecular probes are highly sen sitive for detecting specific food-borne pathogen s, and they have overcome the disadvantages of being time-con suming, tediou s operation and ambiguou s results, which are usually associated with conventional detection methods. Key words ：E nterobacter sakazakii (Cronobacter spp .)；m olecular detect