大白菜DREB类转录因子cDNA的克隆及植物表达载体的构建.pdfVIP

华北农学报2009, 24( 1) : 6973 DREB cDNA 李 妍, 申书兴, 轩淑欣, 李晓峰, 王彦华 ( , 071001) :851 , RTPCR DREBcDNA , BcDREB1(GenBank :E 924266) , BcDREB1 663 bp, 214 ,AP2 ,DREB cDNA pCAMBIA2301 , BcDREB1 pCAMBcDREB1, :;DREB ; ; :Q785 :A : 1000- 7091( 2009)01- 0069- 05 Cloning the cDNA of DREBlike Transcription Factor from Chinese Cabbage and Construction of Plant Expression Vector LI Yan, SHEN Shuxing,X AN Shuxin,LI Xiaofeng,WANG Yanhua (College of Horticulture,Agricultural niversity of Hebei,Baoding 071001,China) Abstract The cDNA of DREB transcription factor named BcDREB1(GenBank accession number E 924266)was ob tained from Chinese cabbage inbred line 851under the drought stress by RTPCR method.The sequence analysis indicated that the clonewas consisted of 663 nucleotides(nt) ,coding 214 amino acids.The deduced primary structure of BcDREB1 contained a singleAP2 domain and showed thetypical characteristics of DREB gene family.A plant expression vector of the BcDREB1 genewas constructed and named pCAM BcDREB1,in which the CDS sequence of BcDREB1 was controlled by the CaMV 35S promoter.Thiswork laid thefoundations for future transgenicresearch on BcDREB1gene function. Key words Chinese cabbage;DREB transcription factor;Gene cloning;Plant expression vector DREB(Dehydration responsive element binding) CBF4, CBF4 CBF/ DREB1 [6] [7] [ 8] DRE() , [ 9] [10] [11] [12] [ 13] [14] , CBF/ DREB , [1,2] [3] Liu , CBF/ DREB 3 ,