两种薄层色谱法鉴别双黄连口服液中黄芩苷_绿原酸的探讨.pdfVIP

China Animal Health 学术研究 两种薄层色谱法鉴别 双黄连口服液中黄芩苷、绿原酸的探讨 程建国，侯丽丽，李常富 （山西省兽药监察所山西太原 030027） 2000 [1] 2005 [2] 摘 要：双黄连口服液标准分别收载于《中国兽药典》 年版 和 年版 二部中，改版收载于 2005 1 2005 年版二部，对【鉴别】项（）方法进行了修订，其他检测项目相同。现按照 年版二部收载双黄连 1 口服液检测产品时，对【鉴别】项（）方法有些见解，提出与大家探讨，通过反复试验，实验结果表明，按 本方法在紫外光灯（254nm）下检视结果时，供试品色谱中无斑点，与对照品色谱相应位置上，未见斑点， 2 365nm 致使无法判定结果，见图 。在紫外光灯（ ）下检视，供试品色谱中，在与对照品色谱相应位置上， R f 4 显相同颜色的荧光斑点，但 值小，分离度不高（见图 ）。 关键词：双黄连口服液；黄芩苷；绿原酸；薄层色谱法 Two Thin- layer Chromatography Methods Identifying Baicalin and Chlorogenic Acid in Shuanghuanglian Oral Liquid Chen J ianguo, Hou Lili, Li Changfu Shanxi Institute of Veterinary Drug Control, Taiyuan, 030027) （ Abstract:The Shuanghuanglian Oral liquid standards were carried in the 2000 edition and 2005 edition of China Veterinary Pharmacopoeia respectively, and in 2005 editon after the correcting. The revision was about “ ” the change of identification item 1,the others were the same. When to test the shuanghuanglian oral with the way of 2005 edition ,some ideas were put forward to discuss. Through the trial and error, the results of this method showed that the test piece chromatograph (254nm) had a spot while the comparison products didnt have. And lead to no way to determine the results (Fig2). In UV265nm, the test