白术对小鼠移植性肉瘤S180的抑瘤作用及对Bcl2基因表达的影响.docVIP

白术对小鼠移植性肉瘤S180的抑瘤作用及对Bcl2基因表达的影响 　　作者：邱根全，赵云，刘昳，蔡云 邱根全 作者单位：1. 西安交通大学医学院第一附属医院中医科，陕西西安 710061;2. 安徽省宣城市中心医院消化内科，安徽宣城 242000 　　【摘要】 目的 探讨白术对小鼠S180肉瘤的抑制作用及对肿瘤凋亡相关基因Bcl2表达的影响。方法 昆明系小鼠60只，皮下注射S180细胞，同时分别给予白术、环磷酰胺处理小鼠，其后2周内测量各组肿瘤生长大小;采用RTPCR法检测凋亡抑制基因Bcl2的表达。结果 白术组的肿瘤重量低于模型组而大于环磷酰胺组(P均0.05)，但白术抑制肿瘤生长的作用强度未呈现量效关系，且其抑瘤率不及环磷酰胺(P0.05);与对照组相比，白术能显著抑制肿瘤Bcl2基因的表达水平(P0.05)。结论 白术具有抑制肿瘤生长的作用，可作为肿瘤治疗的辅助药物。 　　【关键词】 白术;S180肉瘤;Bcl2 　　Baizhu suppresses S180 sarcoma growth and inhibits 　　Bcl2 expression in tumortransfected mice 　　QIU Genquan, ZHAO Yun, LIU Yi, CAI Yun 　　1. Department of Chinese Medicine, the First Affiliated Hospital, Medical School ofXian Jiaotong University, Xian 710061; 2. Department of Internal Medicine,Xuancheng Central Hospital, Xuancheng 242000, China 　　ABSTRACT: Objective To study Baizhus inhibitory effect on S180 sarcoma growth and its effect on Bcl2 expression in tumortransfected mice. Methods S180 cells were subcutaneously injected into 60 healthy Kunming mice. Meanwhile the mice were dealt with MTX or Baizhu, separately; the weight of tumor was measured in the following two weeks; the expression of Bcl2 was detected by RTPCR. Results The tumors weight in Baizhu group was lower than that in model group, but higher than in MTX group (P0.05), but Baizhus inhibition was not associated with its dose (P0.05), and Baizhus rate of tumor growth inhibition was lower than that of MTX. Compared with that in control group, Bcl2 expression was lower in Baizhu group obviously. Conclusion Baizhu can inhibit tumor growth and serve as an adjuvant drug in tumor therapy. 　　KEY WORDS: Baizhu; S180 sarcoma; Bcl2 　　近年来大量研究证实，中医药学在肿瘤防治方面具有不可替代的优势。中药白术的抗肿瘤作用已有很多报道，其作用机制亦涉及多个方面[1]。本研究通过建立移植性S180肉瘤实体小鼠模型,观察中药白术的抑瘤作用，并从RNA水平观察白术对肿瘤凋亡相关基因Bcl2表达的影响，以期对白术的抑瘤机制做更深一步的探讨。 　　1 材料与方法 　　1.1 动物及瘤株 　　健康的昆明系小鼠(雌雄各半，6～7周龄)60只，体重20g左右。肿瘤细胞株：S180肉瘤，腹水型，购自西安交通大学医学院肿瘤研究中心。 　　1.2 方法 　　1.2.1 S180荷瘤小鼠模型及中药白术煎剂的制备 　　S180肿瘤细胞复苏、