霉酚酸对人结肠癌SW620细胞凋亡诱导作用和其机制研究.pdfVIP

霉酚酸对人结肠癌SW620 细胞凋亡诱导作用及其机制研究 栗若兰，郑智慧，路新华，蒋沁，张华 华北制药集团新药研究开发有限责任公司 微生物药物国家研究工程中心，河北 石家庄 050015 摘要：目的：研究霉酚酸(Mycophenolic acid，MPA)对人结肠癌细胞株SW620 凋亡诱导 作用及其机制。方法：MPA 作用SW620 细胞后，MTT 法测细胞增殖抑制活性，Hoechst 33258 染色法检验细胞核形态变化，提取DNA 并电泳检验DNA 断裂情况，流式细胞仪 检测凋亡和细胞周期的影响，并检验细胞中Caspase-3 活性。结果：MPA 有效抑制SW620 细胞的增殖，IC50 为0.11µmol/ L 。经过MPA 处理后的细胞核呈现明显的固缩现象，基 因组DNA 断裂为DNA ladder 。流式细胞仪的测定结果说明MPA 使细胞生长周期停滞 于G1/S 期，并能够浓度、时间依赖诱导SW620 细胞出现典型的sub-G1 凋亡峰。进一 步的细胞 Caspase-3 活性结果，证明MPA 能剂量依赖地诱导细胞中Caspase -3 的活性。 结论：MPA 通过诱导结肠癌细胞的凋亡而抑制细胞的增殖，并且该凋亡诱导作用是通 过激活细胞中Caspase-3 途径。 关键词：霉酚酸、SW620、细胞凋亡、Caspase -3 Apoptosis of Human Colon Cancer Cell Line SW620 Cells Induced by Mycophenolic Acid LI Ruo-lan, ZHENG Zhi-hui, Lu XIN-hua, JIANG Qin, ZHANG Hua National Microbial Medicine Engineering Research Center, New Drug Research and Development Co. , Ltd. of North China Pharmaceutical Corporation, Shijiazhuang 050015, China Abstract: Objective: To study the apoptosis of human colon cancer cell line SW620 induced by Mycophenolic acid and its mechanism. Method: SW620 cells were incubated with Mycophenolic acid for 3 days, the viability of cells were determined using the method of MTT, and the cell nucleus was detected by Hoechst 33258 straining. DNA fragment was detected by DNA electrophoresis. Flow cytometry was used to analyze the cell cycle and apoptosis. The activity of Caspase-3 was measured by Flurescence assay. Results: MPA remarkably inhibited the proliferation of SW620 cell with an IC50 of 0.11µmol/ L. After treatment of MPA, the cell nucleus exhibited characteristic condensation and DNA fragment. Flow cytometry results showed that the cell cycle was arrested at the G1/S phase and the sub-G1 was increased with the con