钛合金表面纳米图案化结构对成骨细胞行为影响.pdfVIP

·178· 田昂，等：钛合金表面纳米图案化结构对成骨细胞行为的影响 中国攀枝花 钛合金表面纳米图案化结构对成骨细胞 行为的影响 田 昂，史晓国，杨 合，薛向欣 (资源循环与环境工程研究所，材料与冶金学院，东北大学,沈阳，辽宁，110819) 摘 要:材料的界面修饰问题是当前再生医学和组织工程领域的核心问题，从材料学角度讲，材料的界面修饰是植 入材料“嵌入”宿主体内组织所需要的纽带；从生物学角度讲，界面修饰能够被用来进行材料表面细胞行为及其相 互作用机理的研究，是调控细胞行为的一种重要手段。本研究通过阳极氧化法对钛及其合金表面进行界面修饰， 制备出一种表面图案化材料,即 TiO2 纳米管阵列涂层。研究发现，成骨细胞在该涂层表面的活性及迁移行为受控于 纳米管的管径尺寸，并展现出一定的尺寸依赖效应，纳米管与黏附蛋白整合素间的相互作用是造成此种效应的主 要因素。 关键词：组织工程；纳米管；成骨细胞；尺寸依赖效应 The Nano-Pattern Structure of Titanium Alloy Control the Behavior of Osteoblast Tian Ang, Shi Xiaoguo, Yang He, Xue Xiangxin (Institution of resources recycling and environment engineering, school of materials and metallurgy, Northeastern University, Shenyang, Liaoning, 110819) Abstract: Interface modification of material was the significant research focus in the fields of regenerative medicine and tissue engineering. From the aspect of materialogy, the modification was the regulator that affects the compatibility of implant; on the aspect of biomaterials, the modification could control the cells behavior and be used to study the interaction between cells and substrate. In this paper, TiO2 nanotube array coatings were fabricated by anodic oxidation method on the substrate of titanium. The analysis indicated that the osteoblast behaviors such as cell viability and migration were controlled by the caliber of nanotube. The interaction between integrin and nanotubes would result in the size dependent effect. Key words: tissue engineering, nanotube, osteoblast, size dependent effect 0 引言 对 TNT 纳米管的管径有明显的尺寸依赖性，但是 关于这种现象的机理却存在着诸多争议。例如，不 结缔组织细胞在纳米管阵列涂层表面孵育时，