粮食、食用植物油中黄曲霉毒素B1测定方法探讨.pdfVIP

粮食、食用植物油中黄曲霉毒素B 测定方法的探讨 薛冰 (云南国家粮食质量监测中心，云南昆明，650033) 摘 要：本文根据国标薄层色谱法，采用仅是点样滴加样式不同分别测定，并结合酶联免疫法进行定性 测定，相互印证的实验方案，对薄层色谱法薄层板制备与选用、玉米与小麦等成分较复杂样品的 提取方法、点样滴加样式作了研究和改进。总结出了用薄层色谱法测定粮食、食用植物油中黄 曲霉毒素B。时，采用点样滴加样式一点法(样液一点法)，具有准确、快捷、安全、经济合理的 特点。 关键词：粮食 食用植物油 黄曲霉毒素B。 薄层色谱法 点样滴加样式 酶联免疫法 Discussionofdeterminationofaflatoxinin B1 grain， edible oil vegetable XueBing NationalGrain’S 650033) (Yunnan QualityInspectionCenter，Kunming ofnational weremeasured standard，the Abstract：Accordingthin—layerchromatography samplepoints with for adifferent immunosorbent bydropping style，combinedenzyme-linked assayqualita— tivedetermination，the confirmeachother，the program thin-layerplateprepa— experimental rationandselectionof methodsof suchas thin—layerchromatography，extractioningredients cornandwheatmore concludethatdeterminationofaflatoxinin complex．We B1 grain，edi— ble oil in isaccurate,fast， vegetablebypoint—likedroppingstylethin-layerchromatography safeandeconomical． words：Grain；edibleoil；aflatoxin dropping Key vegetable B1；thin—layerchromatography；point—like immunosorbent assay style