青枯雷尔氏菌的色谱行为表征及其防效的相关性研究.docVIP

青枯雷尔氏菌的色谱行为表征及其防效的相关性研究 余谦1,2，郑雪芳2，刘波2( （1.大学生命科学学院，福建 福州 350008；2.福建省农业科学院农业生物资源研究所，福建 福州 350003）摘要: 采用高效离子交换色谱法对世界上分布最广，危害最大的植物性致病菌-青枯雷尔氏菌进行色谱表征。弱致病力的青枯雷尔氏菌的纯培养物经过高效液相色谱分离得到三种不同的特征谱图:单一的特征峰、双峰和三峰，其比例分别为54.1%、16.2%、29.7%。强致病力的菌株只在保留时间为17min时出现单一的特征峰。在生防试验中，接入强致病性的青枯雷尔氏菌后的26天，有10株菌的防效达到30%以上，最好的是T838，其防效为70.8%。对弱致病力的青枯雷尔氏菌进行色谱分析，结合21株弱致病性青枯雷尔氏菌的生物防效,结果发现, 10株生防效果较好的青枯雷尔氏菌中，有8株的特征色谱只出现单一的特征峰。得出生物防效较好的青枯雷尔氏菌大多数出现单一的特征峰这一结论。该研究为阐明青枯雷尔氏菌致病力分化，最终筛选优良的生防菌具有重要的意义。 关键词：高效液相色谱法；青枯雷尔氏菌；生防菌 The Ralstonia solanacearum chromatographic behavior characterization and anti-efficiency analysis Yu Qian1,2, Zheng Xuefang2, Liu Bo2* （1College of Life Science university, Fuzhou 350108, China; 2Biotechnology Institution, Fujian Academy of Agricultural Sciences, Fuzhou 350003）Abstract ：Ralstonia solanacearum, a widely distributed pathogen, was characterized by high-performance liquid chromatography (HPLC). The pure culture of Ralstonia solanacearum was successfully separated into one two or three characteristic fractions. The rates were 54.1%, 16.2% and 29.7%. The results showed that strong virulent strains formed only one characteristic peak at the retention time of 17 minute. In the bio-control test, ten Ralstonia solanacearum strains’ control effect more than 30%.The best strain was T838, which reached 70.8%. Twenty-one strains were analyzed by HPLC and applied to the experiment of Biological control effect. The results showed that there are eight better control effect strains formed only one characteristic peak. As the result, we may conclusion that most of better control effect strains formed only one characteristic peak. The results are very important to elucidate the mechanism of Ralstonia solanacearum’S pathogentic mutation and screening excellent bio-control bacteria． Key words：high-performance liquid chromatographyRalstonia solanacearum; bio-control bacteria 青枯雷尔氏菌(Ralstonia solanacearum)，属于革兰氏阴性细菌引起的植物细菌性青枯病害(Bacterial wilt)，简称青枯病，是一种世界范围的细菌性土传病害。该菌寄主广泛，能够侵染热带和温带地区多达450种以