银杏叶提取物对急性缺血再灌注后视网膜神经节细胞的保护作用.pdfVIP

660 ChinOphthalRes，August2009，Vo1．27，No．8 · 实 验 研 究 · 银杏叶提取物对急性缺血再灌注后视网膜神经节细胞 的保护作用 李月华 马 科 徐 亮 TheneurOprOtectiVeeffectofextactofginkobilobaon retinalganglion cells afterretinalischemia-reperfusion LiYuehua，MaKe，uLiang．DepartmentofOphthalmology，BeijingTongrenHospital，Beijing100005，China Abstract Objective Lossofretinalganglioncells(RGCs)isadominantpathologicalfeatureafterelevation of intraocularpressure(IOP)．OurpreviousstudyshowedthatginkgobilobaextracthasaneuroprotectiveeffectonRGCs．Present studywasto investigatetheeffectofextactofginko biloba onRGCssurvivalafteracuteocularhypertention—induced retinal ischemia·reperfusioninjury． Methods Retinalischemia—reperfusionmodelswereinducedbyinfusingnormalsalinesolution intoanteriorchambertoelevateIOPto110mmHgforonehourandthenreducingtheIOPtonormalreadingtoallow reperfusionin therighteyesof60 SD rats．Thenormal~llow eyeswereascontrols．5ml／kgofnormalsalinesolution(NS)，1％ erigeron breviscapus(EB)，0．25％ EGb761，1％ EGb761，4％ EGb761wasadministeredvialavagingat2hourafterinjuryandonceaday afteroperationofr28daysrespectivelyindifferentgroups．RGCswereretrogradelylabeledbyinjectionof3％ fluorogoldintoboth sideofsuperiorcolliculusatthe23thdayafterinjury．Theratsweresacrificedandretinaswerecollectedatthe28thdayafter injury．SurvivalrateofRGCswascalculatedattheratioofRGCsdensitybetweenrighteyesandlefteyes．Animalexperiment followedtheStandardofAssociationofrResearch inVisionandOphthalmology． Results ThemeansurvivalrateofRGCswas 66．58％ ±6．83％ ，75．62％ ±6．9％ ，74．92％ ±6．78％ ，76．57％ ±7．86％ ，79．87％ ±7．49％ in NS group，1％ erigeron breviscapusgroup，0．25％ ，1％ and4％ EGb761grouprespectively，indicatingsignificantdifferenceamongdifferentgroups(F 5．13，P：0．001)．ComparedwithNSgroup，thesurvivalrateof