金银花新品种‘花瑶晚熟’组培快繁技术的研究.pdfVIP

48 金银花新品种‘花瑶晚熟’组培快繁技术研究 蔡 能，王晓明，李永欣，曾慧杰，杨硕知，乔中全 1. 湖南省林业科学院，湖南长沙 410004 ；2. 湖南省林木无性系育种重点实验室，湖南长沙 410004 摘 要：以‘花瑶晚熟’为研究对象，取当年抽生的嫩枝茎段为外植体，研究了不同消毒处理对初代污 染率的影响，分析了不同基本培养基、激素配比对组培苗增殖及生长的影响，探讨了生长素浓度对 组培苗生根的影响，进行了大批量组培苗的移栽。研究结果表明，‘花瑶晚熟’幼嫩茎段的合适消毒方 式以“紫外线 15min+75%乙醇 5s+5%次氯酸钠 7min+0.1%升汞 3min”为最佳；外植体在初代培养基 MS+BA0.5mg/L 上萌发腋芽后，腋芽在改良 DKW4+BA1.0mg/L+NAA0.1mg/L+生物素 2.0mg/L 培养 基上继代培养，组培苗增殖系数达到 4.63 ，组培苗生长势良好；组培苗生根适合培养基为 1/2MS+IBA3.0mg/L+活性炭 200mg/L ，生根率达 97.92% ；在温室大棚中，采用穴盘或平盘，用泥炭 土︰珍珠岩为 1 ︰1 或泥炭土︰珍珠岩︰细河沙为 1 ︰1 ︰1 两种基质对组培苗进行大批量移栽，移栽 成活率达到 96.5% 以上。 关键词：灰毡毛忍冬；‘花瑶晚熟’；组织培养；快速繁殖；增殖；生根；移栽 Studies on Tissue Culture and Rapid Propagation of Honeysuckle cv.‘huayaowanshu’ CAI Neng, WANG Xiao-ming, LI Yong-xin, ZENG Hui-jie , YANG Shuo-zhi, QIAO Zhong-quan (1.Hunan Academy of Forestry，Changsha 410004，Hunan, China ；2. Hunan Key Laboratory of Trees Clones Breeding Technology，Changsha 410004，Hunan, China) Abstract: By taking growing shoots of ‘huayaowanshu’ as testing materials, disinfection efficacy of different disinfection methods was tested, effects of basic medium and plant growth regulators on the proliferation of tissue culture seedlings, the best rooting medium was screened and the plantlets were transplanted. The results showed: “at first, ultraviolet radiation 15min, then 75% alcohol 5s, and then 5%NaClO 7min, at last 0.1%HgCl2 3min” was a suitable disinfection method. The medium used for producing axillary buds from stem segments was MS+ 6-BA 0.5 mg/L. The best proliferation medium was modified DKW4+6-BA1.0mg/L+NAA0.1mg/L+Biotin2.0mg/L, and the multiplication coefficient was 4.63 with strong plantlets. The rooting rate was 97.92% on the medium 1/2MS+IBA3.0mg/L+AC200mg/L.Using aperture disks or flat disks,