SutcliffeJGCompletenucleotidesequenceofthe：SutcliffeJG核苷酸全序列分析.pdfVIP

CC/NUMBER 51 This Week’s Citation Classic ® DECEMBER 17, 1990 Sutcliffe J G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp. 43:77-90, 1979. [Biological Laboratories, Harvard University, Cambridge, MA] This paper describes the nucleotide sequence of Maxam-Gilbert sequencing method in part because pBR322 and the strategy that was developed to ensure I had lobbied for an assessment of its accuracy. I the accuracy of the results. The biological features selected the 3-lactamase gene of pBR322 for study encoded by the plasmid are interpreted through the because it was evident this was a superior doning sequence, as are features that enhance its value as a vector and any information about it would be valu- cloning vector. [The SCI® indicates that this paper able. Over seven months, I learned the method, has been cited in more than 1600 publications, mak- developed strategies for studying an entire gene, ing it the most-cited paper from this journal.[ and scaled-up the technology, espethlly gel electro- phoresis, since then it could require 10 gels to ob- The Sequence of pBR322 tain 100 bases (now it’s kilobases/gel). Every base was critical as errors could alter the apparent triplet I. G