反相高效液相色谱法(RP-HPLC)测定冷却猪肉中生物胺.pdfVIP

江西农业学报2008，20(8)：82—84 Aeta Jiangxi Agrieuhurae 徐振1，孟勇2，朱志远1，徐幸莲1，吴光红2，周光宏h (1．南京农业大学教育部肉品质量控制重点实验室，江苏南京210095，2．江苏省淡水水产研究所，江苏南京210017) 摘要：应用反相高效液相色谱法(RP—HPLC)建立同时测定冷却猪肉中多种生物胺的方法。样品经0．4mol／L高氯酸 提取后用丹磺酰氯柱前衍生，流动相为乙腈和水，采用梯度洗脱，流速为1．0mL／min，荧光检测器激发波长(E】【)340am，发射 波长(Era)515 nm。结果表明：采用该方法色胺、苯乙胺、腐胺、尸胺、组胺、酪胺、亚精胺和精胺8种生物胺能在25min内得到 很好分离。线性范围为0．5—20 关键词：反相高效液相色谱(fuP—HPLC)；生物胺；冷却猪肉 中图分类号：0657．72文献标识码：A文章编号：1001—8581(2008)08一0082—03 Determinationof AnlinesinChilledPorkReverse Biogenic by Performance Phase-—high LiquidChromatography XU Zhenl，MENG Zhi—yuanl，XUX．ing—lian‘，WUGnang—hon92，ZHOU Yon92，ZHU Guang—hon91‘ 0fMeat of (1．1(eyI_aboratoryPl’0cl嘲堍andQualityControl，MinistryEducation，N跚jingAgriculturalUniversity，Nanjing210095，China； 2．FreshwaterResearchInstituteof Fishery JiangsuProvince，N舳jing210017，China) methodof thecontentsof aminesinchilled RP—HPLCwas Abstract：The detecting simultaneously biogenic porkby developed． 0．4 thenderived elution wereextractedmol／L chloride．Themobile Wmaa Samples by HCl04，and usingdamyl phase gradient pro- witha mixtureofacetonitrileand flowratewasI mL／min．FluorescencedetectorWas withEx340am gram binary wate；}．The applied andEra showed of 515姗．Resultsa goodseparationeightbiogenie and 25min．ItWaslinearinthe ofconcentrationsbetween0．5and histamine，tyramine，spermidinespermine)within range 20彬mL from82．43％to97．14％forallamines．TheRSD