沙棘组培快繁技术研究.pdfVIP

, Journal o Anhui Agri. Sci. 2005, 33( 2) : 236- 237, 249 罗芸 罗芸 1 2 1 1 周洁 , 岳冬梅 , 陈贵 , 张立军 ( 1. , 110161; 2. , 118109) 研究了不同取材时间不同基本培养基和不同浓度的6BA 和NAA 组合对沙 组培快繁的影响结果表明: 以春秋季节休眠芽 为外植体获得的沙 组培苗效果最好; B + 6BA0. 5mg/ L+ NAA 0. 5~ 1.0 mg/ L 为适宜的启动培养基, 对休眠芽的诱导率为100% ; 1/ 2B 5 5 + 6BA 0. 5mg/ L+ NAA 0. 5mg/ L 为最佳继代增殖培养基; 1/ 2B + 6BA 0. 1mg/ L+ NAA 1. 0 mg/ L 为最佳生根培养基, 生根率达到85% ; 试 5 管苗经过驯化移栽成活率达50% 以上 沙 ; 组培快繁;6BA;NAA S793. 6 A 0517- 6611(2005) 02- 0236- 02 In Vitro Propagation of Hippophae Rhamnoides ZHOU Jie et al ( College o Biology and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning 110161) Abstract In th is article, the in luence o several actors on micropropagation o Hippophae rhamnoides was investigated.The results showed: B medium 5 containing 0. 5mg/ L 6BA and 0. 5~ 1. 0 mg/ L NAA was suitable or initial culture; the highest rate or shoot propagation were obtained on 1/ 2B medi 5 um supplemented with the same concentration o 6BA( 0. 5mg/ L) and a lower concentration o NAA( 0. 5mg/ L) ; 1/ 2B medium supplemented with 0. 1 5 mg/ L 6BA and 1.0 mg/ L NAA were ound to be the best medium or root initiation and growth; a ter in vitro hardening, the plantlets were transplanted to soil where the plants grew w ell. Key words Hippophae rhamnoides in vitro propagation; 6BA;NAA , , 1/ 2 ; ; B , 6BA NAA 5 , 1. . 5 20~ 25 , 60% ~ 70% 1 , , , , , 1