20140695A-星点设计-响应面法对珠子参水提工艺优化的研究.pdfVIP

中南药学 2014 年 5 月 第 12 卷 第 5 期　 Central South Pharmacy. May 2014, Vol. 12 No. 5 星点设计- 响应面法对珠子参水提工艺优化研究 * 翟思程，唐志书 ，宋忠兴，王景媛（陕西中医学院 / 陕西省中药资源产业化协同创新中心 / 陕西省中药基础与新药研 究重点试验室，陕西 咸阳 712046） 摘要：目的　优化珠子参水提工艺。方法　以竹节参皂苷Ⅳa 含量为指标，以水倍量、提取时间、提取次数 为因素，采用三因素三水平，通过星点设计- 响应面法确定最佳的水提工艺。结果　最佳提取工艺为13 倍量水， 提取3 次，每次90 min 。结论　本工艺虽然采用了水煎煮提取，但对于竹节参皂苷Ⅳa 的提取率与乙醇提取 的结果无显著性差别，并且提高了药物在水中的溶解度，为后续的颗粒剂型的研究提供了理论依据，同时对 药材价格及所用溶媒进行了估算，发现采用水煎煮提取可以大大的节省成本，适用于工业化大生产和新药的 开发。 关键词：星点设计- 响应面法；水提工艺优化；珠子参 中图分类号：RXXX　　　　文献标识码：A　　　　文章编号：1672-2981(2013)03-0000-00 doi:10.7539/j .issn.1672-2981.2013.03.001 Optimization of water extraction process of Panacismajoris by central composite design/response surface methodology * ZHAI Si-cheng, TANG Zhi-shu , SONG Zhong-xing, WANG Jing-yuan (Shaanxi University of Chinese Medicine, Xianyang Shaanxi 712046) Abstract: Objective To optimize the water extraction process of Panacismajoris . Methods Water extraction was used to extract active components.The content of Panax j aponicas Ⅳ a was regarded as evaluation indicator, optimizing the times of water, extraction times and time by central composite design/response surface methodology. Results The optimum technological conditions was extracting was extracting 3 times by 13 times amount of water for 90 min each time. Conclusion Compared with ethanol extraction, there is no significantly difference with decoction of water extract, using decoction of water also can save the cost, avoid organic solvent pollution, and provide a theoretical basis for industrial production and the development of new drugs. Key words: response surface methodology; optimization of water extraction proces; Panacismajoris 　　 珠 子 参 为 五 加 科 植 物 珠 子 参 Panax japonicas C.A. 　　 日立 LC-7000A 双泵 系统液相色谱仪 （日本 日立公 Me