小鼠侵袭性肺曲霉病发病过程中TLRs_NF_B信号通路激活.pdfVIP

TLRs/NF-JB 10 # 881 # doi: 10. 3969/ j. iss . 1000-484X. 2010. 10.004 TLRs/ NF-JB ¹ º º ( 江西中医学院现代中药制剂教育部重点实验室, 南昌330004) R341 A 1000-484X(2010)10-0881-04 [ ] : ( IPA) TLRs/ NF-JB , IPA : ( N) ( N+ Af) IPA ( IPA) , (Af) , Af , RT-PCR TLR2 TLR4 mRNA NF-JB p65IL- 1B : ( 1) Af 72 , IPA , , N+ Af ; ( 2) N+ Af , IPA TLR2 mRNA , TLR4 mRNA , NF-JB p65 , IL-1B :TLRs , Af IPA [ ] TLR/ NF-JB; ; Af; ; The activation of TLRs/ NF-JB signal athways in the invasive ulmonary as- ergillosis of mice LI X iang , L UO H ong-Dan, SHI Qing , XIE X iao-Mei, LI UJin-H ui. Key Laboratory fo M odern Prep aration of Traditional Chine e Medicine, Mini try of Education, Jiangxi Univer ity fo Traditional Chine e Medicine, N anchang 330004, China [Abstract] Objective:To study the activatio of TLRs/ NF-JB sig al pathway i I vasive Pulmo ary Aspergillosis. Methods:The mice were ra domly selected i to three groups:the ormal mice( N) , the ormal mice with i fectio ( N+ Af) a d IPA mice( IPA) .The mice were ad- mi istrated with A p erg illu f umigatu (Af) via i tra asal a d sacrificed at differe t time.The lu g tissue of mice were extracted u der sterile co ditio , a d the studied by pathological sectio , cou ti g the lu g burde a d detecti g the expressio of the TLR2TLR4 mRNA by RT- PCR, NF-JB a d IL-1B with the Wester blot. Results:72hs later after i fectio , IPA mice were serious i flammatory reactio a d hypha ap- peared, also lu g burder was higher tha i N mice. Compared to the N+ Af mice,TLR2 mRNA of IPA mice were high expressio ab ormally at late time with, persiste t low expressio TLR4 mRNA, a d NF-JB p65 i creased sharply early a d the decreased persiste tly, IL- 1B was i low expressio . Conclusion:The ab ormally activatio of TLRs/ NF-JB i duces low actio of th