黄连解毒汤对动脉粥样硬化大鼠MCP_1_CCR2mRNA表达的干预作用.pdfVIP

中药新药与临床药理2010 年11 月第21 卷第6 期 ·583 · 黄连解毒汤对动脉粥样硬化大鼠MCP- 1/CCR2 mRNA 表达的干预作用 1 2 3 郭重仪，黄 萍，李国强 （1.暨南大学药学院，广州510632 ；2.广州中医药大学中药学院，广州 510006 ；3. 广州医学院第二附属医院心内科，广州510260） 摘要：目的 研究黄连解毒汤对动脉粥样硬化大鼠主动脉组织MCP-1/CCR2mRNA表达的影响。方法 建立动 脉粥样硬化大鼠模型，随机分成正常对照组、模型对照组和黄连解毒汤低，中，高剂量组，阿托伐他汀组，用 RT-PCR方法检测各实验组大鼠主动脉组织MCP-1，CCR2 mRNA表达。结果 与模型组相比，黄连解毒汤中， 高剂量组及阿托伐他汀组的MCP-1 mRNA、CCR2 mRNA 表达明显降低，结果有显著性差异（P 0.05～0.01）。 结论 黄连解毒汤能下调MCP-1，CCR2 mRNA在主动脉的表达，减少局部炎症反应，减轻血管的损害。 关键词：黄连解毒汤；动脉粥样硬化；MCP-1 ；CCR2；mRNA表达 中图分类号：R285.5 文献标识码：A 文章编号：1003-9783 （2010）06-0583-04 Effect of Huanglian Jiedu Decoction on the Expression of MCP-1/CCR2 mRNA in Atherosclerosis Rats 1 2 3 GUO Zhongyi ，HUANG Ping ，LI Guoqiang （1. College of Pharmacy， Jinan University Guangzhou 510632 ， China; 2. School of Chinese Herbal Medicine ，Guangzhou University of Chinese Medicine ，Guangzhou 510006 ， China;3. The Second Affiliated Hospital of Guangzhou Medical College ，Guangzhou 510260 ，China） Abstract ：Objective To study the effect of Huanglian Jiedu Decoction on the expression of MCP-1/CCR2 mRNA in rats with atherosclerosis （AS）. Methods After the AS model was established ，AS rats were divided into model group ，low- ， middle- and high-dosage Huanglian Jiedu Decoction groups and atorvastati group． Meanwhile ，a normal control group was also set up. The expression of MCP-1/CCR2 mRNA in aorta was tested by RT-PCR method ． Results Compared with the model group ，the expression of aortic MCP-1/CCR2 mRNA was significantly 1ower in middle- and high-dosage Huanglian Jiedu Decoction groups and in atorvastati group （P 0.05 or P 0.01）. Con－ clusion Huanglian Jiedu Decoction can decrease the expression of MCP-1/CCR2 mRNA in aorta ，reduce local in－ flammatory reaction ，and relieve the aortic damage. Keywords ：Huanglian Jiedu Deco