黑果枸杞果实多糖降血糖生物功效及其机制研究.pdfVIP

244 2009, Vol. 30, No. 05 食品科学 ※营养卫生 汪建红，陈晓琴，张蔚佼 (新疆师范大学生命科学与化学学院，新疆乌鲁木齐 830054) ：目的：探讨黑果枸杞果实多糖的降血糖生物功效及其机制。方法：采用预防性灌胃高、中、低不同 剂量黑果枸杞果实多糖溶液后再造模与先造模再治疗性灌胃高、中、低不同剂量黑果枸杞果实多糖溶液两种方法， 分别与对照组、糖尿病模型组及盐酸二甲双胍组比较，研究了不同浓度的黑果枸杞果实多糖对糖尿病的预防和治疗 作用，并对其血清与肝脏的超氧化歧化酶(superoxide dismutase ，SOD)活性、丙二醛(malon dialdehyde ，MDA)含 量及肝糖原水平等进行了测试，以便了解对黑果枸杞果实多糖的降糖作用机制。结果：黑果枸杞果实多糖能显著 降低糖尿病小鼠的血糖含量，增强糖尿病小鼠血清和肝脏SOD 活性，降低其血清和肝脏MDA 含量，并能促进葡 萄糖转变为肝糖原。结论：黑果枸杞果实多糖有较好的防治糖尿病的作用。虽然其降糖效果弱于盐酸二甲双胍， 但增强小鼠的抗氧化能力的效果要优于盐酸二甲双胍。同时，黑果枸杞果实多糖对糖尿病的多饮多食、体重减轻 症状也有一定缓解作用。 ：黑果枸杞；多糖；小鼠；降血糖功能；作用机制 Study on Hypoglycemic Function of Polysaccharides from Lycium ruthenicum Murr. Fruit and Its Mechanism WANG Jian-hong ，CHEN Xiao-qin ，ZHANG Wei-jiao (College of Life Science and Chemistry, Xinjiang Normal University, Urumuqi 830054, China) Abstract ：In this study, two ways was used to prove and explain the hypoglycemic function of polysaccharides from Lycium ruthenicum Murr. fruit, among which, one way was to preventively administer the normal mice by gavage with the low, middle or high dosages of the polysaccharides for 11 d, and then intraperitoneally inject the prevented mice with alloxan, and another way was to intraperitoneally inject the normal mice with alloxan to establish diabetic models, and then intraperitoneally administer the diabetic mice by gavage with the low, middle or high dosages of the polysaecharides for 21 d. Finally the hepatin in liver, SOD activity and MDA content in serum and liver, and fasting blood glucose level were determined for the control group, diabetic model group, all treatment groups and metformin hydrochloride group. The results showed that the polysaccharides signifi- cantly can reduce glucose level in blood and MDA content in blood