响应面法优化杆菌肽发酵培养基.pdfVIP

第 34 卷第 2 期 Vol.34,No.2 2011 年 2 月 HEBEI HUAGONG Feb.2011 医药化工 响应面法优化杆菌肽发酵培养基 李 欣，黄昌伟 (华北制药集团华胜公司，河北 石家庄 052160) 摘 要：采用响应面分析法对杆菌肽产生菌的发酵培养基进行优化。采用Plackett-Burman实验筛选出影响杆菌 肽产量的主要因素为豆粕、NaSO、生物氮素，利用最陡爬坡路径逼近响应区域，应用Box-Behnken设计和响应面分 2 2 3 析优化得到最佳发酵培养基，发酵单位较优化前提高了36.9%。 关键词：杆菌肽；响应面；优化；培养基 中图分类号：R978.16+ 文献标识码：A 文章编号：1003-5095(2011)02-00017-04 Optimization of Fermentation Medium for Bacitracin Production by Response Surface Methodology LI Xin,HUANG Chang -wei （North China Pharmaceutical Group Corporation Huasheng Company，Shijiazhuang 052160,China） Abstract:The fermentation materials of bacitracin producing strain were optimized by the response surface methodology. First, Plackett-Burman design was adopted to screen out the important factors affecting the output of bacitracin: soybean meal, Na S O , biological nitrogen. Then the path of the steepest ascent was utilized to approach the optimal region. At 2 2 3 last, the optimized medium was obtained by Box-Behnken design and response surface methodology. Under the condi- tion, the fermentation-titer was increased by about 36.9 ％compared with the previous condition. Key words:bacitracin;response surface methods;optimization;medium 杆菌肽是一种短肽类的新型广谱抗菌素,能够 1.2 培养基 + - 固体培养基：蛋白胨10g/L；酵母膏3.0g/L；氯化 有效抑制G 和G细菌,如金黄色葡萄球菌、链球菌 等，其成分为A、B1、B2、B3、D1、D2、D3和E等，其中 钠5g/L；琼脂粉2.0g/L。 [1-3] A成分活性最高，比例最大 。