响应面法优化耐高温酵母生产高浓度乙醇.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2010, January 25; 26(1): 42−47 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2010 CJB, All rights reserved. 工业生物技术 申乃坤，王青艳，陆雁，秦燕，黄日波 530007 : 利用耐高温酵母GXASY-10 菌株对木薯粉同步糖化 (SSF) 法生产高浓度乙醇的发酵条件进行了优化。在单因 素实验的基础上，首先应用Plackett-Burman 试验设计筛选影响酒精高温高浓度发酵的重要参数，采用最陡爬坡实验逼 近最大酒精生产区域后，利用 Box-Behnken 设计确定重要参数的最佳水平。筛选结果表明，影响酒精产量的重要参数 是液化时间、糖化酶用量和初始木薯粉 (底物) 浓度。最佳工艺条件为：液化时间为35 min ，糖化酶添加量为 1.21 AGU/g 底物，底物浓度为 37.62 ％。20 L 发酵罐在此条件下 (发酵温度 37 ℃，转速100 r/min) 经过48 h 发酵，酒精浓度可达 16.07 ％ ( V/ W) 。优化条件与初始条件相比较，酒精浓度提高了33 ％。 : 燃料乙醇，木薯，同步糖化发酵，Plackett-Burman 设计，Box-Behnken 设计 Enhancing ethanol production using thermophilic yeast by response surface methodology Naikun Shen, Qingyan Wang, Yan Lu, Yan Qin, and Ribo Huang National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, Nanning 530007, China Abstract: We optimized the conditions of simultaneous saccharification and fermentation (SSF) from cassava flour into high-concentration ethanol by thermophilic yeast GXASY-10. Based on the single factor experiment, we screened the important parameters by Plackeet-burman design. We used the path of steepest ascent to approach to the biggest region of ethanol production subsequently. Then, we obtained the optimum values of the parameters by Box-Behnken design. The results showed that the important parameters were the liquefaction time, glucosidase dosages and initial concentration of cassava flour (substrate). The optimum technical conditions were as follo