一氧化氮和线粒体ATP敏感性钾通道介导血管紧张素转换酶 抑制剂加强阈下预处理的作用.pdf

Acta Physiologica Sinica, August 25, 2005, 57 (4): 453-460 453 ATP 1 1 1 2 2 2,* 1 2 313000 310006 Langendorff () () (angiotensin-converting enzyme inhibitors, ACEI)(nitric oxide, NO) ATP (mitochondrial ATP-sensitive potassium channel, mitoKATP channel)ACEI (1)2 min 10 min (subthreshold preconditioning, sPC) ( 30 min + 120 min) (2)ACEI sPC sPC (left ventricular end-diastolic pressure, LVEDP) (left ventricular developed pressure, LVDP)(lactate dehydrogenase, LDH)sPC (3)NOS L -NAME mitoKATP 5-HD 10 min / sPC LVEDP LVDP LDH (P 0.05) (4) sPCNO ACEI sPC NO (P 0.05) ACEI NO mitoKAT P ATP Q 4 6 3 Angiotensin-converting enzyme inhibitors potentiate subthreshold precondition- ing through NO and mitoKATP channel 1 1 1 2 2 2,* ZHANG Hong , ZHANG Bei , TANG Bo-Yu , CHEN Ying-Ying , ZHU Li , SHEN Yue-Liang 1 Department of Physiology, Huzhou Teachers College, Huzhou 313000, China; 2Department of Physiology, School of Medicine, Zhejiang University, Hangzhou 310006, China Abstract: The aim of the present study was to determine whether angiotensin-converting enzyme inhibitors (ACEI) could contribute to the protective effects of preconditioning, and to explore its underlying mechanism. The Langendorff model of isolated rat heart was used. Cardiac contractility and lactate dehydrogenase (LDH) in the coronary effluent were measured, and infarct area of hearts after 30 min of ischemia followed by 120 min of reperfusion was analyzed. We found that: (1) The subthreshold preconditioning (2 min of ischemia and 10 min of reperfusion), captopril (an ACEI with sulfhydryl groups) or perindoprilate (an ACEI without sulfh