好氧反硝化菌T7用于废水脱氮特性的研究.pdfVIP

1 3 Vol. 1 No. 3 2008 11 Environment and Ecology in the T hree Gorges Nov. 2008 T7 汤丽娟, 罗固源, 许晓毅, 林晓东, 陈燕云 ( , 400045) : ( SND) OGO , T7, , , , PCR 16SrDNA , 16SrDNA T7 ( Rhodococc us ) , C/ N 5 , ( PVA) , , , : , , PVA : ; ; ; ; PVA :X703 : A :2008) Study on Nitrogen Removal Characteristics of an Aerobic Denitrifying Bacterium Rhodococcus Strain T7 TANG Lijuan, LU O Guyuan, XU Xiaoyi, LIN Xiaodong, CHEN Yanyun ( Key Laboratory of Thr ee Gorges Reservoir Regions EcoEnvironm ent M inistry of Education , Chongqing University, Chongqing 400045, China) Abstract: One strain of aerobic denitrifying bacterium , named as T 7, with high activit y of nitrogen removal w as iso lated from OGO reactor possessing simultaneous nitrification and denitrification ( SND) phenomenon, T7 is Gram positive , rodlike and pink and identif ied as R hodococc us according to its PCR amplification and 16SrDNA sequence homology comparison. It could grow w ell wit h potassium nitrate as only nitrogen source and sodium cit rate as only carbon source, and opt imum rat io of C/ N w as five. The amplification culture of this bacterium w as used in synthet i cal nit rate wastewat er treatment, and polyvinyl alcohol ( PVA) w as selected as entrapment carrier to immobilize T7. The experimental results indicat e that the bacterium can met abolize nit rate w hen sufficient oxygen exists, and can treat different concentrations of nitrogencontaining w astewater . Besides, t he mode of dropping bacteria immobilized by polyvinyl alcohol( PVA) can provide ad