LY294002对胃癌细胞SGC7901增殖和相关调控因子的影响.docVIP

LY294002对胃癌细胞SGC7901增殖及相关调控因子的影响 　　作者：许朝,姜藻　　作者单位：东南大学附属中大医院 肿瘤中心,江苏 南京 　　【摘要】目的:研究PI3K抑制剂LY294002对SGC7901胃癌细胞增殖凋亡及相关调控因子的影响。方法：MTT法测定细胞增殖,流式细胞术Annexin VFITC/PI染色法检测细胞凋亡率,RTPCR检测NAG1、Skp2 mRNA的表达情况。结果：LY294002可抑制SGC7901的生长,呈时间剂量依赖性,细胞表现出形态学改变;经10、30、50 Μmol·L-1的LY294002作用12 h的凋亡率依次为(12.7±0.42)%、(36.5±0.45)%、(64.1±0.79)%,明显高于空白对照组的(2.3±0.36)%(均P0.01);LY294002可上调NAG1 mRNA表达,下调Skp2 mRNA表达。结论：LY294002可改变胃癌细胞株SGC7901 Skp2和NAG1基因的表达,抑制细胞增殖,诱导细胞凋亡,其机制可能与其调控NAG1、Skp2 mRNA表达水平有关。 　　【关键词】 胃癌细胞; PI3K; 细胞凋亡; Skp2; NAG1 　　Influence of PI3K inhibitor on the proliferation and related regulatory 　　factors of gastric carcinoma cell SGC7901 　　XU Chao, JIANG Zao(Center of Clinical Oncology, Zhongda Hospital, Southeast University, Nanjing 210009, China) 　　[Abstract] Objective: To explore the influence of PI3K inhibition by LY294002 on the proliferation and correlated regulatory factors of gastric carcinoma cell SGC7901.Methods： The effect of LY294002 on the proliferation of gastric cancer cell were evaluated by MTT assays.Flow cytometry analysis was used to determine the apoptosis rate. RTPCR was applied to measure the expression of NAG1(Nonsteroidal antiinflammatory drug activated gene1) and Skp2 (Sphase kinase associated protein2)mRNA. Results： Administered with LY294002,the proliferation of gastric cancer cell decreased in dose and timedependent manners.The cell apoptosis rates after the treatment with 10, 30, 50 Μmol·L-1 LY294002 for 12 h were(12.7±0.42)%， (36.5±0.45)%， (64.1±0.79)% respectively, which were higher than those in control group(2.3±0.36)%(P0.01). The expression of Skp2 mRNA was downregulated while that of NAG1 mRNA was upregulated. Conclusion： LY294002 could inhibit proliferation and induce apoptosis of SGC7901 cells, possibly through the regulation of Skp2 and NAG1 expressions. 　　[Key words] gastric carcinoma cell line; PI3K; cell apoptosis; Skp2; NAG1 　　胃癌是我国常见的恶性肿瘤之一。近年来分子生物学研究表明,胃癌的发生与多种癌基因及抑癌基因的异常有关。PI3K/Akt信号