靶向抗肿瘤肽的构建和其生物学活性研究.pdfVIP

Chinese Journal of Biochemical Pharmaceutics 2010 31 1 19 1 2 1 1 马 丽, 蔡在龙, 王庆蓉, 雷呈祥 ( 1. 海军医学研究所, 2. 上海长海医院, 上海 200433) : 构建重组靶向抗肿瘤肽(Citrostatin) , 研究其生物学活性 构建Citrostatin 重组基因, 原核 生物表达和纯化获得Citrostatin 融合蛋白, 通过内皮细胞杀伤实验细胞毒活性实验体外管状机构生成抑制实验等 分析 Citrostatin 的生物学活性 Citrostatin 融合蛋白 表达酶切纯化后, 目的蛋白纯度达90% 以上Citrostatin 能明显抑制内皮细胞ECV304 的增殖(IC = 2. 28 Lmol/ L) , 有效杀伤肿瘤细胞 1990 及NCI H640(IC 分别为 9. 24, 50 50 2.74 Lmol/ L) , 并明显抑制体外管状结构的生成 成功构建了重组靶向抗肿瘤肽Citrostatin, 细胞和血管水平 研究表明该融合肽同时具有抑制肿瘤新生血管的形成和直接杀伤肿瘤的双重活性 : Citrostatin; 融合肽; 抗肿瘤药; 血管生成抑制 : Q78; R962 :A : 1005 1678(2010) 01 0019 04 Study on construction of citrostatin and its bioactivity 1 2 1 1 MA Li , CAI Zai long , WANG Qing rong , LEI Cheng xiang (1. Naval Medical Reseach Institute, 2. ChanghaiHospitalAff iliated to No. 2 Military Medical University, Shanghai 200433, China) Abstract: Purpose To construct a recombined antitumor peptide and to analyze its bioactivity. Methods Constructing a recombined gene and inserting the pGEX 4T 3 vector. The recombined proteinwas expressed in . coli BL21 and purified with Amylose Resin. Then, citrostatin was subjected to the following tests separate ly: inhibition of endothelial cell proliferation,MTT test of cytotoxicity and inhibition of endothelial cell tube for mation on ECMatrix. Results Citrostatin significantly inhibited the proliferation of human endothelial cell ECV304( IC = 2. 28 Lmol/ L) . It also