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Transcription factor genes.ppt
* Transcription factor genes ERF1 ERF2 ERF4 Relative expression At1g33760 0 0.01 0.02 0.03 0.04 0.05 0.06 At1g06160 0 0.1 0.2 0.3 0.4 0.5 0.6 ERF8 0 0.04 0.08 0.12 0.16 MYB75/PAP1 1h 3h 6h 24h 0 0.05 0.1 0.15 0.2 0.25 0.3 WRKY26 0 0.01 0.02 0.03 0.04 WRKY33 0 0.05 0.1 0.15 0.2 ZAT10 0 0.05 0.1 0.15 0.2 0.25 At5g04340 0 0.1 0.2 0.3 ERF13 1h 3h 6h 24h 0 0.002 0.004 0.006 1h 3h 6h 24h TDR1 0 0.01 0.02 0.03 ERF11 0 0.02 0.04 0.06 0.08 0.1 ERF6 0 0.005 0.01 0.015 0.02 0 0.1 0.2 0.3 0.4 0.5 0.6 0 0.2 0.4 0.6 0.8 1 1.2 Col-0 MeJA jin1-9 MeJA Col-0 mock jin1-9 mock Supplemental Figure 1: Q-RT-PCR expression analysis of selected MYC2-regulated genes in jin1-9 vs. Col-0, after MeJA or mock treatment. Q-RT-PCR expression analysis of genes after treatment with 0.1 mM MeJA black symbols or mock white symbols treatment in Col-0 squares and jin1-9 triangles . Data are expressed as relative RNA levels [mRNA]gene/[mRNA]actin and are means of three biological replicates 30 pooled plants each ; error bars denote standard errors. See Methods for further information and Supplemental Table 3 for primer sequences used in the Q-RT-PCR experiments. At3g28740/P450 0 0.005 0.01 0.015 0.02 0.025 0.03 0.035 0.04 1h 3h 6h 24h relative expression Col-0 MeJA jin1-9 MeJA Col-0 mock jin1-9 mock Phenylpropanoid pathway genes Phytohormone biosynthesis genes AOC4 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18 1h 3h 6h 24h relative expression Col-0 MeJA jin1-9 MeJA Col-0 mock jin1-9 mock ACS6 0 0.02 0.04 0.06 0.08 0.1 0.12 1h 3h 6h 24h Relative expression Col-0 MeJA jin1-9 MeJA Col-0 mock jin1-9 mock AACT/At5g61160 0 0.5 1 1.5 2 2.5 3 1h 3h 6h 24h relative expression Col-0 MeJA jin1-9 MeJA Col-0 mock jin1-9 mock 1h 3h 6h 24h *
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