促进金线莲生长发育的内生真菌筛选研究.pdfVIP

促进金线莲生长发育的内生真菌筛选研究 * 王雅俊，孟志霞，于雪梅，王春兰，郭顺星 （中国医学科学院-北京协和医学院药用植物研究所，北京 100193 ） 摘要：目的 筛选促进金线莲生长发育的优良内生真菌。方法 在离体培养条件下，将金线莲试管苗与4 株分离自兰科植物 的内生真菌在5 种供试培养基上共培养，根据菌苗共生特点筛选适宜的双重培养基。在此基础上，将 17 株分离自兰科植物 的真菌与金线莲苗共生培养，以鲜重和增高为指标，筛选可促进金线莲生长发育的内生真菌。结果 17 株真菌中有 1 株显 著促进金线莲苗增高(P ＜0.05) ，3 株极显著促进金线莲苗增高(P ＜0.01) ，最高促增高可达对照的2.04 倍，其中 2 株为金线 莲有益真菌。结论 双重培养基和金线莲有益真菌的筛选为研究内生真菌和金线莲的共生机制及人工栽培金线莲新方法奠定 了基础。 关键词：内生真菌；金线莲；纯培养；筛选 中图分类号：S567 文献标识码：A 文章编号：1001-2494(2009)13-0976-04 Screening of Endophytic Fungi Promoting Growth and Development of Anoectochilus roxburghii * WANG Ya-jun, MENG Zhi-xia, YU Xue-mei, WANG Chun-lan, GUO Shun-xing (Institute of Medicinal Plants Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193,China) ABSTRACT ：OBJECTIVE To select the endophytic fungi promoting growth and development of Anoectochilus roxburghii . METHODS A. roxburgii seedlings and 4 strains endophytic fungi isolated from the orchids were cultured together on five kinds of medium in vitro culture. Suitable dual culture medium were selected according to the growth of the plantlets and fungi . On the basis of these, 17 strains of fungi isolated from orchids were cultured together with seedlings for one month, respectively. Endophytic fungi promoting growth and development of A. roxburgii were selected , with its fresh weight, plant height as index. RESULTS One strain could promote seedlings growth significantly (P0.05), and three strains extremely significantly increased the plant height of A. roxburghii seedlings(P0.01) , which could reach as high as 2.04 times compared with the control. Two strains of them showed effective influence on A. roxburghii growth. CONCLUSION The selectio