Ghrelin对雌激素诱导小鼠胸腺细胞凋亡的抑制作用.pdfVIP

对成年雄性金色中《 Ghrelin对雌激素诱导小鼠胸腺细胞 凋亡的抑制作用 李 江 尹 帆 马勇江 张 媛 李 英 李玉谷※ 华南农业大学兽医学院，广州510642 摘 要：方法：6周龄BALB/C雌性小鼠80只，分为4组：雌激素+Ghrelin组、雌激素+生理盐水组、生理盐水组、空白 对照组。雌激素+Ghrelin组首先隔日腹腔注射苯甲酸雌二醇 （0.1mg/只）2周，然后采用相同方法注射Ghrelin(120μg/ 只)2周；雌激素+生理盐水组首先注射苯甲酸雌二醇2周，然后注射生理盐水2周；生理盐水组注射生理盐水4周;空 白对照组不注射任何试剂。之后，测量各组小鼠的胸腺指数，同时应用光镜、电镜和流式细胞术检测胸腺的显微结 构、超微结构和胸腺细胞凋亡率。结果：雌激素+Ghrelin组的胸腺指数极显著大于雌激素+生理盐水组，胸腺细胞凋 亡率极显著小于雌激素+生理盐水组(p＜0.01)，而二者与生理盐水组和空白对照组均差异不显著(p＞0.05)；雌激素 +Ghrelin组的胸腺显微结构和超微结构基本恢复正常，并与生理盐水组和空白对照组相似。结论：Ghrelin可以通过抑 制胸腺细胞凋亡和促进胸腺细胞增殖，从而逆转雌激素诱导的小鼠胸腺萎缩。 关键词:Ghrelin;雌激素；细胞凋亡；胸腺；小鼠 InhibitoryeffectofGhrelinontheestrogen-inducedthymocyteapoptosisinmice LIJiangYINFanMAYong-jiangZHANG YuanLIYu-gu* CollegeofVeterinaryMedicine,SouthChinaAgriculturalUniversity,Guangzhou510642,China Abstract:Methods:80Six-week-oldfemaleBALB/Cmiceweredividedinto4groups:the estrogen+Ghrelingroup,theestrogen+salinegroup,thesalinegroup,andthecontrolgroup.Inthe estrogen+Ghrelingroupmicefirstlywereinjectedintraperitoneallywithestradiolbenzoate(0.1mg/per) everyotherdayfor2weeks,thentreatedwithGhrelin(120lμg/per)for2weeksinthesameway;inthe estrogen+salinegroupmicefirstlywereadministratedwithestradiolbenzoatefor2weeks,thensalinefor 2weeks;inthesalinegroupmicewereinjectedwithsalinefor4weeks;inthecontrolgroupmicewere outofbeingtreated.Finally,thymusindex,microstructure,ultrastructureandthymocyteapoptosisratio ofthymusweredeterminedbyusinglightmicroscopy,electronmicroscopyandflowcytometry.Results: thethymusindexintheestrogen+Ghrelingroupisextremelyhigherthanthatintheestrogen+saline group,whilethethymocyteapoptosisratiointheestrogen+Ghrelingroupisextremelylowerthanthatin theestrogen+salinegroup(p＜0.01),buttheyhavenosignificantdifferencescomparedwiththesaline groupandthecontrolgroup (p＞0.05);andthemicrostructureandultrastructureofthymusinthe estrogen+Ghrelingroupalmostreturnedtonormal,resembletothatinthesalinegroupa