Dickkopf_DKK1_在肝癌组织和多种人肿瘤细胞系中的表达分析.pdfVIP

2006 12 26 12 T umor Vol. 26, No. 12, Dec. 2006 # 1109 # Clinical Research # 临床研究 Dickkopf ( DKK 1) 1, 2 1, 2 1, 2 2 2 2 2* 余艳军 , 万晓桢 ,于 彬 , 游海燕 ,舒惠群 ,姚根富 , 覃文新 ( 1. , 200032; 2. , 200032) [ ] 目的: 检测dickkopf ( DK K 1) 在肝癌组织及多种恶性肿瘤细胞系中的表达情况方法: 采用酶联免疫吸附 验 real time PCRRT-PCR 及Western blot 对D 1在肝癌组织及多种恶性肿瘤细胞系的表达情况进行检测结果:肝癌组织 检测RT-PCR, 10/ 15 肝癌中高表达;Real-time PCR 方法检测, 8/ 8肝癌中高表达;Western blot 方法检测,5/ 8 肝癌中高表达 酶联免疫吸附 验检测到D 1 在多种恶性肿瘤细胞系的细胞培养液上清液中均有表达, 蛋白质表达量波动于5~ 210 ng/ mL 之间; 10 种人恶性肿瘤细胞系在mRNA 水平和蛋白质水平D 1的表达均能检测到结论: DK K 1高表达于肝癌组织 及广泛表达于多种恶性肿瘤细胞系, 提示 D 1 可能在肿瘤的发生发展中起到重要作用 [ ] 癌, 肝细胞; 基因,DK K 1; 肿瘤细胞,培养的 [ ] R735. 7 [ ] A [ ] 1000-7431(2006) 12-1109-04 Expression of Dickkopf (DKK 1) in liver cancer and in multiple human cancer cell lines 1, 2 1, 2 1, 2 2 2 2 2* YU Yan-jun ,WAN Xiao-zhen , YU Bin , YOU Ha-i yan , SHU Hu-i qun , YAO Gen-fu , QIN Wen-xin ( 1. Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. National ey Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200032, China) [ABSTRATC] O jective:To detect the expression of dickkopf (DK K 1) in liver cancer and in multiple human cancer cell lines. Methods: The expression of D 1in liver cancer and in multiple human cancer cell lines was detected by enzyme-linked immu- nosorbant assay (ELISA) , reverse transcriptase-polymerase chain reaction ( RT-PCR), rea-l time PCR, and Western blotting. Results: Sem-i quantitative PCR demonstrated that the expression of DK K 1 was up-regulated in 10 out of 15 hepatocellular carci- noma ( HCC) tissues. Rea-l timePCR sho