骨桥蛋白与尿激酶型纤溶酶原激活物在喉鳞状细胞癌中的表达和临床意义.docVIP

骨桥蛋白与尿激酶型纤溶酶原激活物在喉鳞状细胞癌中的表达及临床意义 　　作者：张中华，梁昆，马超，雷大鹏，潘新良 作者单位：1. 山东大学齐鲁医院耳鼻咽喉-头颈外科， 2. 山东大学卫生部耳鼻喉科学重点实验室， 济南 250012 　　【摘要】 目的 探讨骨桥蛋白(OPN)和尿激酶型纤溶酶原激活物(uPA)在喉鳞状细胞癌组织中的表达及其与喉癌侵袭、转移的关系。方法 应用免疫组织化学方法检测64例喉癌组织、18例癌旁正常黏膜中OPN和uPA的表达，从部位、分化程度、临床分期、淋巴结转移等不同方面分别进行对照分析，比较其有无差异。 结果 64例喉癌组织中OPN与uPA蛋白的阳性表达率分别为62.5%(40/64)、65.6%(42/64)。OPN与uPA的表达与肿瘤组织临床病理分期和有无淋巴结转移有关(P0.05)，与患者年龄、性别、肿瘤的部位及分化程度等无关(P0.05)，且二者表达呈正相关(r=0.6625,P0.05)。 结论 OPN和uPA基因蛋白在喉癌的侵袭转移过程中起着重要作用，有望成为反映喉癌生物学特性及评价预后的重要标记分子。 　　【关键词】 喉肿瘤;骨桥蛋白;尿激酶型纤溶酶原激活物;免疫组化;侵袭转移 　　ZHANG Zhonghua1, LIANG Kun1, MA Chao2, LEI Dapeng1, PAN Xinliang1 　　(1. Department of Otorhinolaryngology Head and Neck Surgery, Qilu Hospital of Shandong University, Jinan 250012, China; 　　2. Key Laboratory of Otorhinolaryngology, Ministry of Health, Shandong University, Jinan 250012, China) 　　To investigate expressions of osteopontin (OPN) and urokinasetype plasminogen activator(uPA) and their relation to the invasiveness and metastasis of squamous cell carcinoma of the human larynx. Methods Expressions of OPN and uPA in 64 cases of laryngeal carcinoma tissue and 18 cases of adjacent normal tissue were detected by using an immunohistochemistry method. Differences of age, sex, differentiation degree, clinical stage and lymphatic metastasis were analyzed. Results In 64 cases of laryngeal carcinoma tissues, the positive expression rates of OPN and uPA were 62.5%(40/64) and 65.6% (42/64) respectively ,which were obviously higher than those in adjacent normal tissues(P0.05).　Expressions of OPN and uPA in carcinoma tissues were related to clinical pathological stage and lymphatic metastasis(P0. 05), but not related to age, sex or differentiation degree (P0. 05). Expression of OPN was positively correlated with that of uPA in laryngeal carcinoma tissues(r=0.6625, P0.05). Conclusion Expressions of OPN and uPA can reflect biological characteristics of laryngeal carcinoma and could be used as important molecular markers in the prognosis