骨肉瘤组织中骨桥蛋白、COX-2与VEGF表达.docVIP

骨肉瘤组织中骨桥蛋白、COX-2和VEGF表达 　　作者：廖有乔,李锋,熊敏,刘家国,张劲松,徐圣康 　　[摘要]　目的:探讨骨肉瘤组织中骨桥蛋白(OPN)、环氧化酶-2(COX-2)和血管内皮细胞生长因子(VEGF)表达的临床意义。方法:应用免疫组化方法检测38例骨肉瘤组织中OPN、COX-2和VEGF蛋白表达情况。结果:OPN、COX-2和VEGF蛋白总阳性率分别为60.53%，63.16%, 68.42%, 在不同恶性级别的骨肉瘤中均具有差异性(P0.05)，而且与肿瘤的恶性程度相关;COX-2，OPN与VEGF表达正相关。结论:OPN，COX-2和VEGF在肿瘤的进展过程中起促进作用,OPN和COX-2是VEGF表达调控因子。OPN可能是肿瘤形成过程中的早期分子事件,可作为监测肿瘤恶性进展的一个生物学标志。 　　[关键词]　免疫组化;血管生成;骨肉瘤; COX-2;VEGF;OPN 　　Expression of Osteopontin,COX-2 and VEGF in Osteosarcoma 　　LIAO You-qiao，LI Feng, XIONG Min，LIU Jia-guo，ZHANG Jin-song，XU Sheng-kang. 　　(Department of Orthopaedic,Taihe Hospital,Yunyang Medical College,Shiyan，Hubei 442000;Department of Orthopaedic,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan，Hubei 443007;Department of Orthopaedic,Dongfeng Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China) 　　Abstract:Objective　To observe the expression of OPN,COX-2 and VEGF in osteosarcoma.Methods The expression of Osteopontin,COX-2 and VEGF in 38 cases with osteosarcoma was examined with immunohistochemistry.Results　The total positive rate of OPN,COX-2 and VEGF was 60.53%,63.16%, 68.42%,respectively.There was significant difference among different malignant osteosarcoma (P0.05),and the expression was correlated with the malignant degree.VEGF expression was positively correlated with the expression of OPN and COX-2. Conclusion OPN,COX-2 and VEGF play a role in the pathogenesis of osteosarcoma,OPN and COX-2 are regulatory factors of VEGF expression.OPN expression may be an early molecular event in the process of tumorigenesis and could be used as a biological marker to monitor the malignant process of osteosarcoma. 　　Key words:Immunohistochemistry;Angiogenesis;Osteosarcoma;COX-2;VEGF;OPN 　　骨肉瘤是最常见的恶性骨肿瘤，恶性度极高，预后差，目前其发生发展的过程尚不清楚，临床上也缺乏特异性的病理诊断指标。有研究认为骨桥蛋白(osteopontin，OPN)、环氧化酶-2(cycloxygenase-2，COX-2)和血管内皮细胞生长因子(vascular endothelial growth factor，VEGF)与一些恶性肿瘤的发生有关，并可作为肿瘤诊断及判断预后的辅助指标[1-5]，但其与骨肉瘤的关系尚不十分清楚。