一种肺脾两虚型慢阻肺动物模型建立_张伟.pdfVIP

# 142# 辽宁中医杂志 2009年第 36 卷第 1期 1 1 2 1 1 1 1 , , , , , , ( 1. 广州中医药大学第一附属医院, 广东 广州 510405; 2. 中山大学附属第一医院, 广东 广州 510080) : 目的:寻求建立肺脾两虚型慢性阻塞性肺病( COPB)大鼠模型的新方法方法: 用熏香烟 30天联合两次气 内滴入脂多糖 ( PS 2001Lg /次)建立肺虚型 COPD大鼠模型, 并配合番泻叶泻下法建立脾虚型动物模型, 从而建立肺 脾两虚型 COPD大鼠模型结果:模型组动物有慢性支气 炎阻塞性肺气肿的特征性病理改变, 有肺脾两虚的症状体 征表现; 气道壁厚度高于正常对照组(P 0101)结论: 烟熏法联合气 内滴入脂多糖并配合番泻叶泻下法可以成功建 立肺脾两虚型 COPD大鼠模型 : 慢性阻塞性肺疾病; 动物模型; 大鼠 : R- 332 : A : 1000- 1719( 2009) 01- 0142- 02 A New way ofEstablishing aRatChronicObstructivePulmonary DiseasewithDeficiency of bothLungand Spleen odel 1 1 2 1 1 1 1 ZHAN G Wei, GONG Jing , ZHAN G Jing-xuan , X IAO H ui-zhen , SUN Zh i-j ia , LIU Jian-bo , XIE Hu i-w en (11 he F irstAff iliatedHosp ital of Guangzhou University of CM, Guangzhou 510405, Guangdong, China; 21 he F irstAff iliatedH osp ital of Zhongshan University, Guangzhou 510080, Guangdong, China) Abstract:Objective: To find a new way of establish ing a rat chronic obstructive pulm onary disease( COPD)w ith deficiency of both lung and spleen m ode.lM ethods: The m odel group w as established by intratracheal instillation of lipopolysaccharide( PS) tw icew ith exposure to cigarite sm oke abou t 30 days. And then the ratsw ere treatedw ith gastric infusion of senna leaf steep. W e compared themodel groupw ith the control group, through observing the general state and the changes in h istopathology, m easuring the th ickness of airw ayw all. Results: Them odel group had the h istopathological changes of chronic bronchitls and emphysem a. The rats had the symptom s and sign of deficiency of both lung and sp