雷公藤红素对转基因小鼠原代肝细胞HBV抑制作用.pdfVIP

雷公藤红素对转基因小鼠原代肝细胞HBV的抑制作用 张欣蕊1，一，易学瑞2，袁有成2，李娜L2，孔祥平2 (1南方医科大学，广州510515；2解放军第四五八医院全军肝病中心，广州510600) [摘要]目的：研究雷公藤红素对HBV转基因小鼠原代肝细胞的抗HBV效果。方法：改良的两步灌流法分离HBV 转基因小鼠原代肝细胞：MTT法检测细胞毒性作用；药物作用24h后取上清，分别应用ELISA法和荧光定量PCR法 随着雷公藤红素浓度的增加，对ltBsAg和itBV—DNA的抑制作用增强。结论：雷公藤红素在原代肝细胞水平不仅能够 抑制HBV-DNA的复制，而且可以有效的抑制HBsAg的表达。 [关键词]雷公藤红素；乙型肝炎病毒；蛋白酶体抑制剂；转基因小鼠原代肝细胞；HBV—DNA effectofCelastrolon of Inhibitory primaryhepatocytes HBV mice transgenic ZHANGXin．rui 1’。，YIXue．mi2，YUAN Nal’。，KoNG You-chen92，LI Xiang—pin92 SouthemMedical TheLiverCenter (1 510515，China；2 ofPLA，NO．458 University,Guangzhou Hospital of 510600，China) PLA，Guangzhou To theanti-HBVeffectofcelastrolon isolatedfromHBV [Abstract]Objectiveinvestigate primaryhepatocytes mice．MethodsThe were from micewiththemodified transgenic primaryhepatocytesseparatedHBV-Tg two-stepsmethod， the weretreatedwith theuntreatedcellsusedasthecontr01．The ofcelastrol primaryhepatocytes celastrol，with cytotoxicity onthe was MTT thenthe of andHBV-DNAinthe primaryhepatocytesanalyzedby assay,andchangesHBsAg supernatant afterthe treatmentfor24hoursweredeterminedwith andreal-PCR．ResultsThe ofcelastrolon drug ELISA TC50 primary was concentrationsbelowerthan showedno 0．8I_tmol‘L～．celastrol hepatocytes6．49士0．8pmol’L～．At significantcytotoxicity to andHBVoDNAofthecellstreatedwithcelastrolwasde